Recombinant Human Clathrin, Light Chain B, His-tagged

• Cat.No.: CLTB-6968H
• Product Overview: Recombinant human CLTB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-211aa
• Description: CLTB belongs to the clathrin light chain family. Clathrin is a protein that plays a major role in the formation of coated vesicles. It forms a triskelion shape composed of three clathrin heavy chains and three light chains. When the triskelia interact they form a polyhedral lattice that surrounds the vesicle. Coat-proteins, like clathrin, are used to build small vesicles in order to safely transport molecules between cells.
• Form: Liquid. In 20 mM Tris-HCl buffer, pH8.0, 10% glycerol, 0.2mM PMSF, 100mM NaCl.
• Molecular Mass: 25.6 kDa (234aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher).
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMADDFGF FSSSESGAPE AAEEDPAAAF LAQQESEIAG IENDEGFGAP AGSHAAPAQP GPTSGAGSED MGTTVNGDVF QEANGPADGY AAIAQADRLT QEPESIRKWR EEQRKRLQEL DAASKVTEQE WREKAKKDLE EWNQRQSEQV EKNKINNRAS EEAFVKESKE ETPGTEWEKV AQLCDFNPKS SKQCKDVSRL RSVLMSLKQT PLSR
• Purity: >85% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: CLTB clathrin, light chain B [ Homo sapiens ]
• Official Symbol: CLTB
• Synonyms: CLTB; clathrin, light chain B; clathrin, light polypeptide (Lcb); clathrin light chain B; Lcb; clathrin, light chain (Lcb); LCB
• Gene ID: 1212
• mRNA Refseq: NM_001834
• Protein Refseq: NP_001825
• MIM: 118970
• UniProt ID: P09497
• Chromosome Location: 5q35.2
• Pathway: Arf1 pathway, organism-specific biosystem; Bacterial invasion of epithelial cells, organism-specific biosystem; Bacterial invasion of epithelial cells, conserved biosystem; Endocrine and other factor-regulated calcium reabsorption, organism-specific biosystem; Endocrine and other factor-regulated calcium reabsorption, conserved biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem
• Function: peptide binding; protein binding; structural molecule activity