Active Recombinant Mouse PIM3, GST-tagged

• Cat.No.: PIM3-1457M
• Product Overview: Recombinant full-length mouse PIM3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: PIM3 or pim-3 oncogene belongs to a family of protooncogenes that encode serine/threonine protein kinases. Pim-3 expressed in hepatocellular carcinoma development and plays a main role in the proliferation of human hepatoma cell lines. Pim-3 also expressed in human pancreatic cancer cell and phosphorylates BAD to block Bad-mediated apoptosis in human pancreatic cancer cell lines (3).
• Source: Sf9 Cells
• Species: Mouse
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 785 nmol/min/mg
• Molecular Mass: ~63 kDa
• Protein length: Full length
• Purity: >90%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: Pim3 proviral integration site 3 [ Mus musculus ]
• Official Symbol: PIM3
• Synonyms: PIM3; proviral integration site 3; serine/threonine-protein kinase pim-3; KID-1/kinase induced by depolarization; Kid1; BC026639; MGC27707; MGC37517
• Gene ID: 223775
• mRNA Refseq: NM_145478
• Protein Refseq: NP_663453
• Pathway: PluriNetWork, organism-specific biosystem
• Function: ATP binding; kinase activity; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; transferase activity; transferase activity, transferring phosphorus-containing groups