Recombinant Human POU2AF1, His-tagged

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Recombinant Human POU2AF1, His-tagged

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Cat. No. : POU2AF1-3570H
Product Overview : Recombinant human POU2AF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography (1-256aa).
Description : POU2AF1, also known as BOB1, is a lymphocyte specific transcription coactivator protein. This protein interacts only with the Oct1/2 proteins through sub domains in the POU domain of the Oct1/2 proteins, enhancing their transcriptional efficacy. Although having no intrinsic capacity for DNA binding, POU2AF1 associates tightly with the octomer motif in the presence of Oct1 and Oct2. POU2AF1 is expressed at highest levels in spleen and peripheral blood leukocytes.
Form : Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol
Molecular Mass : 29.6 kDa (276aa), confirmed by MALDI-TOF (molecular weight on SDS-PAGE will appear higher)
Purity : > 85 % by SDS-PAGE
Concentration : 0.5 mg/ml (determined by Bradford assay )
Sequences of amino acids : MGSSHHHHHH SSGLVPRGSH MLWQKPTAPE QAPAPARPYQ GVRVKEPVKE LLRRKRGHAS SGAAPAPTAV VLPHQPLATY TTVGPSCLDM EGSVSAVTEE AALCAGWLSQ PTPATLQPLA PWTPYTEYVP HEAVSCPYSA DMYVQPVCPS YTVVGPSSVL TYASPPLITN VTTRSSATPA VGPPLEGPEH QAPLTYFPWP QPLSTLPTST LQYQPPAPAL PGPQFVQLPI SIPEPVLQDM EDPRRAASSL TIDKLLLEEE DSDAYALNHT LSVEGF
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
PDBrendering based on 1cqt. :

Gene Name : POU2AF1 POU class 2 associating factor 1 [ Homo sapiens ]
Official Symbol : POU2AF1
Synonyms : POU2AF1;POU class 2 associating factor 1; OBF1; BOB-1; OCT-binding factor 1; OCA-B; B-cell-specific coactivator OBF-1; POU domain class 2, associating factor 1; OBF-1; BOB1; OCAB; POU domain, class 2, associating factor 1; POU domain class 2-associating factor 1
Gene ID : 5450
mRNA Refseq : NM_006235
Protein Refseq : NP_006226
MIM : 601206
UniProt ID : Q16633
Chromosome Location : 11q23.1
Function : DNA binding; protein binding; transcription coactivator activity; transcription cofactor activity

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