Active Recombinant Rat Epha4 protein, hFc-tagged

• Cat.No.: Epha4-7439R
• Product Overview: Recombinant Rat Epha4 protein(D3ZZK3)(Met1-Tyr547), fused with hFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Rat
• Tag: hFc
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized mouse EFNA5-His at 10 μg/ml (100 μl/well) can bind Rat EPHA4-Fc, The EC50 of Rat EPHA4-Fc is 19.8-46.3 ng/ml.
• Molecular Mass: The recombinant rat EPHA4/Fc is a disulfide-linked homodimer. The reduced monomer comprises 769 amino acids and has a predicted molecular mass of 85.3 kDa. The apparent molecular mass of the protein is approximately 98 kDa in SDS-PAGE under reducing conditions.
• Protein Length: Met1-Tyr547
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Epha4 Eph receptor A4 [ Rattus norvegicus ]
• Official Symbol: Epha4
• Synonyms: EPHA4; Eph receptor A4; RGD1560587
• Gene ID: 316539
• mRNA Refseq: NM_001162411
• Protein Refseq: NP_001155883