Recombinant Mouse Tnfrsf4 protein, Fc-tagged

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Recombinant Mouse Tnfrsf4 protein, Fc-tagged

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Cat.No. : Tnfrsf4-7400M
Product Overview : Recombinant Mouse Tnfrsf4 protein (Val20-Pro211) was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).
Description : Tumor necrosis factor receptor superfamily member 4 (TNFRSF4) is also known as ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, CD antigen CD134, OX40. OX40/TNFRSF4 contains four TNFR-Cys repeats. TNFRSF4 is receptor for TNFSF4/OX40L/GP34 and can interacts with TRAF2, TRAF3 and TRAF5.
Source : HEK293
Species : Mouse
Tag : human IgG1 Fc
Predicted N Terminal : Val 20
Form : Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 47.9 kDa. The protein migrates as 70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Val20-Pro211
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of Tnfrsf4-7400M :

Mouse OX40, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA of Tnfrsf4-7400M :

Immobilized Mouse OX40 Ligand, His Tag at 0.5 μg/mL (100 μL/well) can bind Mouse OX40, Fc Tag with a linear range of 10-16 ng/mL.

Gene Name : Tnfrsf4
Official Symbol : Tnfrsf4
Synonyms : Ox40; ACT35; CD134; Ly-70; Txgp1; TXGP1L
Gene ID : 22163
mRNA Refseq : NM_011659.2
Protein Refseq : NP_035789.1
UniProt ID : P47741

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