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Recombinant Renilla Luciferase protein, His-tagged

Cat.No.: Luciferase-09R
Product Overview: Recombinant Renilla Luciferase fused with His tag at C-terminal was expressed in E. coli.
Description: Luciferase played an important role in many functions.
Source: E. coli
Species: Renilla
Tag : His
Form: Lyophilized. Renilla luciferase protein was constituted in a 0.2 µm filtered phosphate-buffered saline (1x PBS) before lyophilized.
Molecular Mass: 30 kDa
Purity: >95%
Applications: ELISA, Western blotting, Dot Blotting, Immunogen, Protein Array, Function Assay, etc.
Using the Renilla Lucifearse assay kit (Promega), the measured net Relative Light Unit (RLU) was approximately 5 X 10^10 RLU/µg of recombinant enzyme protein.
Storage: Upon arrival, the lyophilized protein may be stored for 2 weeks at 4 centigrade. For long term storage, it is recommended to store desiccated below -20 centigrade in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 centigrade. For long term storage, it is recommended to make appropriate aliquots and store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Reconstitution: Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.1~ 1 mg/mL with sterile ice-cold deionized water or 1x Phosphate Buffered Saline (PBS). This solution can then be further diluted into other aqueous buffers.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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