|Product Overview:||Recombinant Renilla Luciferase fused with His tag at C-terminal was expressed in E. coli.|
|Description:||Luciferase played an important role in many functions.|
|Form:||Lyophilized. Renilla luciferase protein was constituted in a 0.2 µm filtered phosphate-buffered saline (1x PBS) before lyophilized.|
|Molecular Mass:||30 kDa|
|Applications:||ELISA, Western blotting, Dot Blotting, Immunogen, Protein Array, Function Assay, etc.
Using the Renilla Lucifearse assay kit (Promega), the measured net Relative Light Unit (RLU) was approximately 5 X 10^10 RLU/µg of recombinant enzyme protein.
|Storage:||Upon arrival, the lyophilized protein may be stored for 2 weeks at 4 centigrade. For long term storage, it is recommended to store desiccated below -20 centigrade in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 centigrade. For long term storage, it is recommended to make appropriate aliquots and store at -80 centigrade. Avoid repeated freeze-thaw cycles.|
|Reconstitution:||Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.1~ 1 mg/mL with sterile ice-cold deionized water or 1x Phosphate Buffered Saline (PBS). This solution can then be further diluted into other aqueous buffers.|