Recombinant Firefly Luciferase, His tagged, Protein G Labeled

• Cat.No.: Luciferase-04F
• Product Overview: Protein G labeled recombinant firefly luciferase with His tag was expressed in E. coli.

Specification

• Species: Firefly
• Source: E.coli
• Tag: His
• Conjugation/Label: Protein G
• Description: Antibody Binding Firefly Luciferase is a Protein G-Firefly Luciferase (protein G-Fluc) fusion protein. It consists of protein G and firefly luciferase (Fluc), while the two proteins connect via a short peptide. Protein G tightly binds to IgG molecules from rabbit, human, mouse, goat, sheep, cow, and rat. In addition, protein G conjugates are especially valuable for detecting antibodies that bind poorly to protein A, such as human IgG3, mouse IgG1, and immunoglobulins from rat, sheep, and horse. Firefly luciferase (Fluc) is a light-emitting enzyme to estimate gene expression. It catalyzes the oxidation of firefly luciferin, allowing its detection by a luminometer. As an antibody binding luciferase, protein G-Fluc can avoid harmful labeling of the detecting antibody. Because staining protocols vary with application, the appropriate dilution of Antibody Binding Firefly Luciferase should be determined empirically.
• Form: Liquid
• Molecular Mass: 85.3 kDa
• Biotin binding Site: 0
• Purity: ≥ 90% by SDS-PAGE
• Applications: In vitro research use only
• Storage: Short Term Storage: -20 centigrade Long Term Storage: -20 centigrade Avoid freeze/thaw cycle. Aliquot upon arrival.
• Storage Buffer: 50 mM sodium phosphate,100 mM NaCl, 1 mM DTT, pH7.2, 50% glycerol
• Concentration: 0.5-1.0 mg/mL
• Shipping: Cold packs

Gene Information

• Official Symbol: Luciferase
• Synonyms: Luciferase; Luciferin 4-monooxygenase; EC:1.13.12.7
• UniProt ID: Q27757