||Recombinant human ARG1, fused to His-tag at C-terminus, was expressed inE.coliand purified by using conventional chromatography techniques.MW = 35.8kDa (330aa).
||Arginase is a manganese-containing enzyme which catalyzes the hydrolysis of arginine to ornithine and urea. It is the final enzyme of the urea cycle. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The type II isoform has been implicated in the regulation of the arginine/ornithine concentrations in the cell. It is located in mitochondria of several tissues in the body, with most abundance in the kidney and prostate.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 2mM DTT, 100mM NaCl.
||> 85% by SDS – PAGE.
||0.5 mg/ml (determined by Bradford assay).
|Sequences Of Amino Acids:
||MSAKSRTIGI IGAPFSKGQP RGGVEEGPTV LRKAGLLEKL KEQECDVKDY GD LP F ADIPN DSPFQIVKNP RSVGKASEQL AGKVAEVKKN GRISLVLGGD HSL AIGS IS G HARVHPDLGV IWVDAHTDIN TPLTTTSGNL HGQPVSFLLK EL KGKIPDVP GF SWVTPCIS AKDIVYIGLR DVDPGEHYIL KTLGIKYFSM TEV DR LGIGK VME ET LSYLL GRKKRPIHLS FDVDGLDPSF TPATGTPVVG GLTYREGLYI TE EIYKTGLL S GLDIMEVNP SLGKTPEEVT RTVNTAVAIT LACFGLAREG NH KP IDYLNP PKL EH HHHHH.
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.