Active Recombinant Human ACVR2A, His tagged

• Cat.No.: ACVR2A-3819H
• Product Overview: Recombinant Human ACVR2A (NP_001607.1) extracellular domain (Met 1-Pro 134), fused with a polyhistidine tag at the C-terminus, was produced in Human Cell.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met 1-Pro 134
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation.
  The ED50 for this effect is typically 0.4-3 µg/mL in the presence of 10 ng/mL recombinant Activin A.
• Molecular Mass: The recombinant human ACVR2A comprises 126 amino acids and predicts a molecular mass of 14.9 kDa. As a result of glycosylation, the apparent molecular mass of the protein is approximately 35-40 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: ≥ 97 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: ACVR2A activin A receptor, type IIA [ Homo sapiens ]
• Official Symbol: ACVR2A
• Synonyms: ACVR2A; activin A receptor, type IIA; activin A receptor, type II , ACVR2; activin receptor type-2A; ACTRII; ACVR2
• Gene ID: 92
• mRNA Refseq: NM_001616
• Protein Refseq: NP_001607
• MIM: 102581
• UniProt ID: P27037