Active Recombinant Human CA5A protein, His-tagged

• Cat.No.: CA5A-1142H
• Product Overview: Active Recombinant Human CA5A protein(NP_001730.1) (Ala 40-Ser 305) was expressed in E. coli, fused with an Met at N-terminus and a polyhistide tag at the C-terminus.

Specification

• Species: Human
• Source: E. coli
• Tag: His
• Protein Length: 40-305 aa
• Form: Lyophilized from sterile 50mM NaAc, 50mM NaCl, 0.05% Brij 35, pH 5.0.
• Bio-activity: Measured by its esterase activity. The specific activity is >500 pmoles/min/μg.
• Molecular Mass: The recombinant human CA5A consisting of 277 amino acids and has a calculated molecular mass of 31.6 kDa. It migrates as an approximately 33 kDa band in SDS-PAGE under reducing conditions.
• Purity: > 96 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CA5A carbonic anhydrase VA, mitochondrial [ Homo sapiens ]
• Official Symbol: CA5A
• Synonyms: CA5A; carbonic anhydrase VA, mitochondrial; CA5; carbonic anhydrase 5A, mitochondrial; CAV; CAVA; CA-VA; carbonic dehydratase; carbonate dehydratase VA; carbonic anhydrase V, mitochondrial
• Gene ID: 763
• mRNA Refseq: NM_001739
• Protein Refseq: NP_001730
• MIM: 114761
• UniProt ID: P35218