Active Recombinant Human CD40LG (108-261aa)
Cat.No. : | CD40LG-019E |
Product Overview : | Recombinant Human CD40LG (Glu108Leu261, with an Nterminal Met) was expressed in E. coli and produced using nonanimal reagents in an animalfree laboratory. |
- Specification
- Gene Information
- Related Products
Cat. No. : | CD40LG-019E |
Description : | CD40 ligand, CD40L (also known as CD154, TRAP or gp39), is a 261 amino acid type II transmembrane glycoprotein belonging to the TNF family, CD40L is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, like NK cells, mast cells, basophils and eosinophils. The receptor of CD40L is CD40, a type I transmembrane glycoprotein belonging to the TNF receptor family. CD40 is expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelium. Although all monomeric, dimeric and trimeric forms of soluble CD40L can bind to CD40, the trimeric form of soluble CD40L has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members. CD40L mediates a range of activities on B cells including induction of activationassociated surface antigen, entry into cell cycle, isotype switching and Ig secretion and memory generation. |
Source : | E. coli |
Nterminal Sequence Analysis : | Met |
Molecular Mass : | 16.9 kDa (monomer) |
SDSPAGE : | 18 kDa, reducing condition |
Activity : | Measured in a cell proliferation assay using B cellenriched peripheral blood mononuclear cells (PBMC) in the presence of IL4. The ED50 for this effect is typically 1 3 µg/mL in the presence of 20 ng/mL of rhIL?4. |
Endotoxin Leve : | <1.0 EU per 1 μg of the protein by the LAL method. |
Purity : | >97%, by SDSPAGE under reducing conditions and visualized by silver stain. |
Formulation : | Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and EDTA. See Certificate of Analysis for details. |
Reconstitution : | Reconstitute at 0.2 mg/mL in sterile PBS. |
Shipping : | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Storage : | Use a manual defrost freezer and avoid repeated freezethaw cycles. 1. 12 months from date of receipt, 20 to 70 °C as supplied. 2. 3 months, 2 to 8 °C under sterile conditions after reconstitution. |
Unitprot ID : | P29965 |
Gene Name : | CD40LG CD40 ligand [ Homo sapiens ] |
Official Symbol : | CD40LG |
Synonyms : | CD40LG; CD40 ligand; IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L; CD40-L; OTTHUMP00000024130; OTTHUMP00000024131; CD40 antigen ligand; T-cell antigen Gp39; T-B cell-activating molecule; TNF-related activation protein; tumor necrosis factor (ligand) superfamily member 5 |
Gene ID : | 959 |
mRNA Refseq : | NM_000074 |
Protein Refseq : | NP_000065 |
MIM : | 300386 |
Chromosome Location : | Xq26 |
Pathway : | Adaptive Immunity Signaling; Allograft rejection; CD40/CD40L signaling; Calcineurin-regulated NFAT-dependent transcription in lymphocytes; Cell adhesion molecules (CAMs); Inflammatory Response Pathway; Systemic lupus erythematosus; T cell receptor signaling pathway |
Function : | CD40 receptor binding; cytokine activity; tumor necrosis factor receptor binding |
Products Types
◆ Recombinant Protein | ||
CD40LG-197H | Recombinant Human CD40LG Protein, C-His-tagged | +Inquiry |
CD40LG-0818H | Recombinant Human CD40LG Protein, GST-Tagged | +Inquiry |
CD40LG-323H | Active Recombinant Human CD40LG Protein (Met1-Leu261), C-His tagged, Animal-free, Carrier-free | +Inquiry |
CD40LG-196H | Recombinant Human CD40LG Protein, C-His-tagged | +Inquiry |
CD40LG-637M | Recombinant Mouse CD40LG Protein, Fc-tagged | +Inquiry |
◆ Lysates | ||
CD40LG-1205RCL | Recombinant Rat CD40LG cell lysate | +Inquiry |
CD40LG-001CCL | Recombinant Canine CD40LG cell lysate | +Inquiry |
CD40LG-1080CCL | Recombinant Cynomolgus CD40LG cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionYes, there are clinical trials investigating the modulation of CD40LG in autoimmune diseases, exploring its potential role in regulating immune responses that contribute to autoimmunity.
Yes, research suggests that CD40LG modulation might be a viable strategy in the development of treatments for infectious diseases by enhancing immune responses.
The interaction between CD40LG and CD40 is crucial for the activation of B cells, leading to the production of antibodies and the development of an effective immune response.
CD40LG is involved in modulating inflammatory responses by influencing the activation and function of immune cells, contributing to the regulation of the immune system.
CD40LG is being investigated as an adjuvant in vaccine development, aiming to enhance the immune response and improve the efficacy of vaccines against various diseases.
Customer Reviews (3)
Write a reviewThe reliable performance of the CD40LG protein in ELISA and its compatibility with protein electron microscopy structure analysis make it an excellent choice for a wide range of research studies.
Its reliable and accurate performance ensures dependable results, contributing to the advancement of scientific knowledge in various fields of study.
Their knowledgeable and responsive team assists in troubleshooting and finding solutions to any experimental challenges that may arise.
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