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Recombinant Human CD40LG (108-261aa)

Cat. No.: CD40LG-019E
Product Overview: Recombinant Human CD40LG (Glu108­Leu261, with an N­terminal Met) was expressed in E. coli and produced using non­animal reagents in an animal­free laboratory.
Description: CD40 ligand, CD40L (also known as CD154, TRAP or gp39), is a 261 amino acid type II transmembrane glycoprotein belonging to the TNF family, CD40L is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, like NK cells, mast cells, basophils and eosinophils. The receptor of CD40L is CD40, a type I transmembrane glycoprotein belonging to the TNF receptor family. CD40 is expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelium. Although all monomeric, dimeric and trimeric forms of soluble CD40L can bind to CD40, the trimeric form of soluble CD40L has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members. CD40L mediates a range of activities on B cells including induction of activation­associated surface antigen, entry into cell cycle, isotype switching and Ig secretion and memory generation.
Source: E. coli
N­terminal Sequence Analysis: Met
Molecular Mass: 16.9 kDa (monomer)
SDS­PAGE: 18 kDa, reducing condition
Activity: Measured in a cell proliferation assay using B cell­enriched peripheral blood mononuclear cells (PBMC) in the presence of IL­4. The ED50 for this effect is typically 1 ­ 3 µg/mL in the presence of 20 ng/mL of rhIL?4.
Endotoxin Leve: <1.0 EU per 1 μg of the protein by the LAL method.
Purity: >97%, by SDS­PAGE under reducing conditions and visualized by silver stain.
Formulation: Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and EDTA. See Certificate of Analysis for details.
Reconstitution: Reconstitute at 0.2 mg/mL in sterile PBS.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage: Use a manual defrost freezer and avoid repeated freeze­thaw cycles. 1. 12 months from date of receipt, ­20 to ­70 °C as supplied. 2. 3 months, 2 to 8 °C under sterile conditions after reconstitution.
Unitprot ID: P29965
Gene Name: CD40LG CD40 ligand [ Homo sapiens ]
Official Symbol: CD40LG
Synonyms: CD40LG; CD40 ligand; IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L; CD40-L; OTTHUMP00000024130; OTTHUMP00000024131; CD40 antigen ligand; T-cell antigen Gp39; T-B cell-activating molecule; TNF-related activation protein; tumor necrosis factor (ligand) superfamily member 5
Gene ID: 959
mRNA Refseq: NM_000074
Protein Refseq: NP_000065
MIM: 300386
Chromosome Location: Xq26
Pathway: Adaptive Immunity Signaling; Allograft rejection; CD40/CD40L signaling; Calcineurin-regulated NFAT-dependent transcription in lymphocytes; Cell adhesion molecules (CAMs); Inflammatory Response Pathway; Systemic lupus erythematosus; T cell receptor signaling pathway
Function: CD40 receptor binding; cytokine activity; tumor necrosis factor receptor binding

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