Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Active Recombinant Human CD40LG (108-261aa)

Cat.No. : CD40LG-019E
Product Overview : Recombinant Human CD40LG (Glu108­Leu261, with an N­terminal Met) was expressed in E. coli and produced using non­animal reagents in an animal­free laboratory.
  • Specification
  • Gene Information
  • Related Products
Cat. No. : CD40LG-019E
Description : CD40 ligand, CD40L (also known as CD154, TRAP or gp39), is a 261 amino acid type II transmembrane glycoprotein belonging to the TNF family, CD40L is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, like NK cells, mast cells, basophils and eosinophils. The receptor of CD40L is CD40, a type I transmembrane glycoprotein belonging to the TNF receptor family. CD40 is expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelium. Although all monomeric, dimeric and trimeric forms of soluble CD40L can bind to CD40, the trimeric form of soluble CD40L has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members. CD40L mediates a range of activities on B cells including induction of activation­associated surface antigen, entry into cell cycle, isotype switching and Ig secretion and memory generation.
Source : E. coli
N­terminal Sequence Analysis : Met
Molecular Mass : 16.9 kDa (monomer)
SDS­PAGE : 18 kDa, reducing condition
Activity : Measured in a cell proliferation assay using B cell­enriched peripheral blood mononuclear cells (PBMC) in the presence of IL­4. The ED50 for this effect is typically 1 ­ 3 µg/mL in the presence of 20 ng/mL of rhIL?4.
Endotoxin Leve : <1.0 EU per 1 μg of the protein by the LAL method.
Purity : >97%, by SDS­PAGE under reducing conditions and visualized by silver stain.
Formulation : Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and EDTA. See Certificate of Analysis for details.
Reconstitution : Reconstitute at 0.2 mg/mL in sterile PBS.
Shipping : The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage : Use a manual defrost freezer and avoid repeated freeze­thaw cycles. 1. 12 months from date of receipt, ­20 to ­70 °C as supplied. 2. 3 months, 2 to 8 °C under sterile conditions after reconstitution.
Unitprot ID : P29965
Gene Name : CD40LG CD40 ligand [ Homo sapiens ]
Official Symbol : CD40LG
Synonyms : CD40LG; CD40 ligand; IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L; CD40-L; OTTHUMP00000024130; OTTHUMP00000024131; CD40 antigen ligand; T-cell antigen Gp39; T-B cell-activating molecule; TNF-related activation protein; tumor necrosis factor (ligand) superfamily member 5
Gene ID : 959
mRNA Refseq : NM_000074
Protein Refseq : NP_000065
MIM : 300386
Chromosome Location : Xq26
Pathway : Adaptive Immunity Signaling; Allograft rejection; CD40/CD40L signaling; Calcineurin-regulated NFAT-dependent transcription in lymphocytes; Cell adhesion molecules (CAMs); Inflammatory Response Pathway; Systemic lupus erythematosus; T cell receptor signaling pathway
Function : CD40 receptor binding; cytokine activity; tumor necrosis factor receptor binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (5)

Ask a question
Are there any ongoing clinical trials targeting CD40LG for autoimmune diseases? 11/01/2022

Yes, there are clinical trials investigating the modulation of CD40LG in autoimmune diseases, exploring its potential role in regulating immune responses that contribute to autoimmunity.

Can CD40LG be a potential target for the treatment of infectious diseases? 02/27/2021

Yes, research suggests that CD40LG modulation might be a viable strategy in the development of treatments for infectious diseases by enhancing immune responses.

How does the interaction between CD40LG and its receptor CD40 influence antibody production? 11/20/2020

The interaction between CD40LG and CD40 is crucial for the activation of B cells, leading to the production of antibodies and the development of an effective immune response.

How is CD40LG involved in the regulation of inflammatory responses? 08/26/2020

CD40LG is involved in modulating inflammatory responses by influencing the activation and function of immune cells, contributing to the regulation of the immune system.

In what ways is CD40LG being explored for its potential in vaccine development? 04/20/2020

CD40LG is being investigated as an adjuvant in vaccine development, aiming to enhance the immune response and improve the efficacy of vaccines against various diseases.

Customer Reviews (3)

Write a review
Reviews
10/17/2022

    The reliable performance of the CD40LG protein in ELISA and its compatibility with protein electron microscopy structure analysis make it an excellent choice for a wide range of research studies.

    01/01/2022

      Its reliable and accurate performance ensures dependable results, contributing to the advancement of scientific knowledge in various fields of study.

      11/16/2020

        Their knowledgeable and responsive team assists in troubleshooting and finding solutions to any experimental challenges that may arise.

        Ask a Question for All CD40LG Products

        Required fields are marked with *

        My Review for All CD40LG Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends