| Cat. No. : |
DKK1-4318H |
| Description : |
DKK1 is part of the dickkopf family. DKK1 is a secreted protein with 2 cysteine rich regions and takes part in embryonic development through its inhibition of the WNT signaling pathway. High levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. Induction of DKK1 contributes to the pathological cascade triggered by beta-amyloid and is significantly involved in the development of tau phosphorylation. DKK1 reduces the differentiation of osteoblastic precursor cells. DKK1 is an important member in multiple myeloma bone disease. |
| Source : |
Human, HEK |
| Host : |
HEK293-F Cells. |
| Form : |
The DKK1 protein was lyophilized from a 0.2µm filtered solution containing no additives or preservatives. |
| Purity : |
Greater than 95.0% as determined by SDS-PAGE. |
| Bio-activity : |
IC50 = 0.4 to 1.2 nM, determined by the concentration dependent inhibition of human MMP-2 hydrolysis of a peptide substrate (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-(3-[2, 4-dinitrophenyl]-L-2, 3-diaminopropionyl)-Ala-Arg-NH2, under conditions in which recombinant pro-MMP-2 is activated, and the peptide substrate concentration is 5µM. |
| Physical Appearance : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Solubility : |
It is recommended to reconstitute the lyophilized DKK1 in sterile 18Mμ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions |
| Amino acid sequence : |
CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA. |
| Storage : |
Lyophilized DKK1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DKK1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Pathways : |
Wnt signaling pathway |
