Active Recombinant Human EREG protein(Val63-Leu108), hFc-tagged

• Cat.No.: EREG-562H
• Product Overview: Recombinant Human EREG (NP_001423.1) (Val63-Leu108) was expressed in HEK293, fused with the Fc region of Human IgG1 at the N-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: N-hFc
• Protein length: Val63-Leu108
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 2-8 μg/mL.
• Molecular Mass: The recombinant human EREG/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 306 amino acids and has a predicted molecular mass of 33.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhEREG/Fc monomer is approximately 37 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EREG epiregulin [ Homo sapiens ]
• Official Symbol: EREG
• Synonyms: EREG; epiregulin; proepiregulin; ER
• Gene ID: 2069
• mRNA Refseq: NM_001432
• Protein Refseq: NP_001423
• MIM: 602061
• UniProt ID: O14944