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Active Recombinant Human IRE1 protein, His&GST-tagged

Cat.No. : ERN1-1124H
Product Overview : Recombinant Human IRE1 protein(O75460-1)(Pro465-Leu977), fused with His tag and GST tag, was expressed in Insect Cells.
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Source : Insect Cells
Species : Human
Tag : His&GST
Form : Supplied as sterile 20mM Tris, 500M NaCl, 25% glycerol, 0.1mM EDTA, 0.5mM TCEP, 2mM GSH, pH 7.5.Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hard copy of CoA.
Bio-activity : The specific activity was determined to be 5 nmol/min/mg using MBP as substrate
Molecular Mass : The recombinant human ERN1/GST chimera consists of 750 amino acids and has a calculated molecular mass of 86 kDa. It migrates as an approximately 90 kDa band in SDS-PAGE under reducing conditions.
Proteinlength : Pro465-Leu977
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 80 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Publication :
Regulated IRE1α-dependent decay (RIDD)-mediated reprograming of lipid metabolism in cancer (2022)

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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