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Active Recombinant Mouse Interleukin 15

Cat.No. : Il15-484M
Product Overview : Recombinant Mouse Il15 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 115 amino acids and having a molecular mass of 13,321 Daltons.
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  • Gene Information
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Cat. No. : Il15-484M
Description : Interleukin (IL)-15 is a pleiotropic pro-inflammatory cytokine that is expressed in several inflammatory disorders, including rheumatoid arthritis, psoriasis and pulmonary inflammatory diseases. Il15 promotes activation of T cells, neutrophils and macrophages, and is critical to dendritic cell function in several model systems. Recent emerging data suggest that Il15 may serve as a useful therapeutic target across a range of disease states.
Species : Mouse
Source : E.coli
Form : Recombinant Il15 was lyophilized from a concentrated (1mg/ml) solution with 10mM NaP pH 8.0.
Purity : Purity Greater than 95% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
Bio-activity : Recombinant mouse Il15 is fully biologically active when compared to standards. The ED50, as determined by dose-dependent induction of CTLL-2 cell proliferation, is 2.5 ng/ml.
Physical Appearance : Sterile Filtered white lyophilized (freeze-dried) powder.
Solubility : Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mgμs/ml, which can be further diluted into other aqueous solutions.
Purity Greater than 95% as determined by : Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
Protein Content determined by : UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Endotoxin Level : Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Amino acid sequence : MNWIDVRYDL EKIESLIQSI HIDTTLYTDS DFHPSCKVTA MNCFLLELQV ILHEYSNMTL NETVRNVLYL ANSTLSSNKN VAESGCKECE ELEEKTFTEF LQSFIRIVQM FINTS
Storage : Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathways : Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway
Gene Name : Il15 interleukin 15 [ Mus musculus ]
Official Symbol : Il15
Synonyms : Il15; interleukin 15; AI503618
Gene ID : 16168
mRNA Refseq : NM_008357
Protein Refseq : NP_032383
UniProt ID : P48346
Chromosome Location : 8 C2; 8 38.0 cM
Function : Cytokine activity; Cytokine receptor binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What techniques differentiate IL15 and IL2 receptor binding kinetics? 10/22/2022

Techniques discern IL15 and IL2 receptor binding kinetics.

What experimental methods study IL15's involvement in autoimmune diseases? 06/23/2022

Methods explore IL15's role in autoimmune disease mechanisms.

What are the applications of IL15 knockout models in deciphering its functions? 04/26/2022

IL15 knockout models provide insights into its diverse functions.

How do you analyze IL15's impact on memory T cell differentiation? 09/19/2021

IL15's influence on memory T cell differentiation investigated.

How does immunofluorescence microscopy visualize IL15 localization in cells? 07/26/2021

Immunofluorescence microscopy visualizes IL15 subcellular localization.

What approaches validate IL15's role in inflammatory responses? 02/04/2021

Approaches validate IL15's involvement in inflammatory responses.

Could you explain the role of IL15 in NK cell cytotoxicity assays? 10/26/2019

IL15's role in NK cell cytotoxicity analyzed using assays.

Can you detail the use of ELISA in quantifying IL15 secretion? 02/03/2018

ELISA measures IL15 secretion accurately.

How is IL15's effect on regulatory T cells quantified using qPCR? 06/08/2017

qPCR quantifies IL15's impact on regulatory T cells.

How does flow cytometry assess IL15-induced T-cell proliferation? 05/26/2017

Flow cytometry quantifies IL15-driven T-cell proliferation.

Customer Reviews (3)

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Reviews
04/19/2018

    Mapping protein- interactions in DNA transcription for gene regulation.

    02/20/2018

      Suitable for cytotoxicity assays.

      10/22/2017

        Effective in NK cell activation.

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