Recombinant Human IL29 protein, His-tagged

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Recombinant Human IL29 protein, His-tagged

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Cat.No. : IL29-381H
Product Overview : Recombinant Human IL29 (Met1-Thr200) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
Description : Interleukin-29, is a secreted protein which belongs to the IL-28/IL-29 family, and is a type III interferon. It is also known as cytokine Zcyto21, Interferon lambda-1, IFN-lambda-1, IFNλ1, IL-29 and IFNL1, and is highly similar in amino acid sequence to the IL-28, the other type III interferon. IL28A, IL28B, and IL29, also named interferonλ2 (IFNλ2), IFNλ3, and IFNλ1, respectively, are classII cytokine receptor ligands that are distantly related to members of the IL 10 family (11-13% aa sequence identity) and the type I IFN family (15-19% aa sequence identity). Human IL29 cDNA encodes a 200 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and a 181 aa mature protein, which is a monomer in solution. It shares 67% and 69% aa sequence identity with human IL28A and IL28B, respectively. IL-29 plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses and also may play a role in antiviral immunity. Interleukin-29/IL-29 up-regulates MHC class I antigen expression. It is a Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway.
Source : HEK293
Species : Human
Tag : His
Predicted N Terminal : Met1
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 21.1 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Met1-Thr200
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of IL29-381H :

Human IL-29, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Gene Name : IL29
Official Symbol : IL29
Synonyms : IL29; interleukin 29 (interferon, lambda 1); interleukin 29; interleukin-29; IFNL1; IL 29; IFN-lambda-1; cytokine Zcyto21; interferon lambda-1; interferon-lambda-1; interferon, lambda 1; IL-29
Gene ID : 282618
mRNA Refseq : NM_172140
Protein Refseq : NP_742152
MIM : 607403
UniProt ID : Q8IU54
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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