Recombinant Human Interleukin 29 (Interferon, Lambda 1), His-tagged

• Cat.No.: IL29-222H
• Product Overview: Recombinant Human interleukin 29 DNA sequence encoding the signal peptide from human CD33 was fused to the carboxylterminally his-tagged mature human IL-29 (Gly 20 - Thr 200). The chimeric protein was expressed in a mouseNSO Cells.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Description: Interleukin-29 (IL-29), also known as IFN-lambda1, is a protein of the helical cytokine family and is a type III interferon. It is also known as IFNλ1 and is highly similar in amino acid sequence to the IL-28, the other type III interferon. IL-29 stimulates antiviral responses through a heterodimeric receptor. It plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses.
• Molecular Weight: The predicted molecular weight of Recombinant Human IL-29 is Mr 21.4 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 26-35 kDa.
• State Of Matter: Lyophilized.
• Formulation: This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
• Purity: > 95% by SDS Page and analyzed by silver stain.
• Endotoxin: < 1.0 EU/µg as determined by the LAL method.
• Biological Activity: The biological activity of Human IFN-Lambda1 was determined in an anti-viral assay using human HepG2 cells infected with encephalomyocarditis. The expected ED50 for this effect is typically 1 - 5 ng/mL.
• Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Gene Information

• Gene Name: IL29 interleukin 29 (interferon, lambda 1) [ Homo sapiens ]
• Synonyms: IL29; IL-29; IFNL1; IL-28A; IL-28B; IFN-lambda-1; ZCYTO21; interferon-λ2 (IFN-λ2); IFN-λ3; and IFN-λ1; Cytokine ZCYTO21; Interferon lambda-1; interferon, lambda 1; interleukin 29; interleukin 29 (interferon, lambda 1)
• Gene ID: 282618
• mRNA Refseq: NM_172140
• Protein Refseq: NP_742152
• UniProt ID: Q8IU54
• Chromosome Location: 19q13.13
• MIM: 607403
• Pathway: Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway
• Function: cytokine activity; interleukin-28 receptor binding