Recombinant Human Interleukin 9 IL9-476H

Recombinant Human Interleukin 9

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Recombinant Human Interleukin 9

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Cat. No. : IL9-476H
Product Overview : Recombinant Human IL-9 produced in E.coli is a single, non-glycosylated single polypeptide chain containing 127 amino acids and having a molecular mass of 14,004 Daltons.
Description : Interleukin (IL-9) is known to regulate many cell types involved in T-helper type 2 responses classically associated with asthma, including B- and T-lymphocytes, mast cells, eosinophils and epithelial cells. Growing evidence obtained from human genomic analysis and antigen-challenged transgenic mice suggests that IL-9 is a candidate factor in immunoglobulin E (IgE) production and thus is thought to be associated with bronchial inflammation and bronchial hyperresponsiveness (BHR).
Species : Human
Source : E.coli
Form : Lyopohilized from 10 mM NaP, pH 7.5.
Purity : Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
Bio-activity : IL-9 is fully biologically active when compared to a standard. The ED50 as determined by the dose-dependent proliferation of human MO7e cells is < 0.5 ng/ml.
Physical Appearance : Sterile Filtered white lyophilized (freeze-dried) powder.
Solubility : Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mgμs/ml, which can be further diluted into other aqueous solutions.
Purity Greater than 98% as determined by : Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
Protein Content determined by : UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Endotoxin Level : Endotoxin level is <0.01ng/ug or <0.1EU/ug.
Amino acid sequence : MQGCPTLAGI LDINFLINKM QEDPASKCHC SANVTSCLCL GIPSDNCTRP CFSERLSQMT NTTMQTRYPL IFSRVKKSVE VLKNNKCPYF SCEQPCNQTT AGNALTFLKS LLEIFQKEKM RGMRGKI
Storage : Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathways : Asthma; Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway
Gene Name : IL9 interleukin 9 [ Homo sapiens ]
Official Symbol : IL9
Synonyms : IL9; interleukin 9; P40; HP40; IL-9; p40 T-cell and mast cell growth factor; T-cell growth factor p40; p40 cytokine; homolog of mouse T cell and mast cell growth factor 40; OTTHUMP00000159423
Gene ID : 3578
mRNA Refseq : NM_000590
Protein Refseq : NP_000581
MIM : 146931
UniProt ID : P15248
Chromosome Location : 5q31.1
Function : cytokine activity; cytokine receptor binding; growth factor activity

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