| Cat. No. : |
LEP-264O |
| Source : |
Ovine |
| Host : |
Escherichia coli. |
| Form : |
The protein was lyophilized from a concentrated (0.65mg/ml) solution with 0.003mM NaHCO3. |
| Purity : |
Greater than 95.0% as determined by: (a) Gel filtration analysis. (b) Analysis by SDS-PAGE. |
| Bio-activity : |
Pegylated triple antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Its in vitro activity is 6-8 fold lower than the non-pegylated antagonist but in vivo it has profound weight gain effect (as compared to the non-pegylated antagonist like in mouse leptin antagonists), resulting mainly from increased food intake. |
| Physical Appearance : |
White lyophilized (freeze-dried) powder. |
| Solubility : |
It is recommended to reconstitute the lyophilized Leptin-Antagonist Triple Mutant Ovine Recombinant in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8, not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Protein content : |
Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.2 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). |
| Storage : |
Lyophilized Leptin Antagonist Triple Mutant Ovine Recombinant although stable at room temperature for several weeks, should be stored C. Upon reconstitution atμdesiccated below -18 > 0.1 Lep-tA mutant mg/ml and C or evenμup to 2mM and filter sterilization Leptin mutant can be stored at 4 room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles. |
