||Recombinant human MAPK11 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
||MAPK11 (Mitogen-activated protein kinase 11) is a member of the MAP kinase family. This kinase is most closely related to p38 MAP kinases(MAPKs). MAPKs are activated primarily in response to inflammatory cytokines and cellular stress, and inhibitors which target the MAPK14 and MAPK11 have shown potential for the treatment of inflammatory disease. MAPK11 has been shown to interact with HDAC3 and Promyelocytic leukemia protein. This kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment, by cytokines, or by environmental stress.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 100mM NaCl
||43.8kDa (387aa), confirmed by MALDI-TOF
||> 90% by SDS - PAGE
||1mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MGSMSGPRAG FYRQELNKTV WEVPQRLQGL RPVGSGAYGS VCSAYDARLR QKVAVKKLSR PFQSLIHARR TYRELRLLKH LKHENVIGLL DVFTPATSIE DFSEVYLVTT LMGADLNNIV KCQALSDEHV QFLVYQLLRG LKYIHSAGII HRDLKPSNVA VNEDCELRIL DFGLARQADE EMTGYVATRW YRAPEIMLNW MHYNQTVDIW SVGCIMAELL QGKALFPGSD YIDQLKRIME VVGTPSPEVL AKISSEHART YIQSLPPMPQ KDLSSIFRGA NPLAIDLLGR MLVLDSDQRV SAAEALAHAY FSQYHDPEDE PEAEPYDESV EAKERTLEEW KELTYQEVLS FKPPEPPKPP GSLEIEQ
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||ATF-2 transcription factor network; Activated TLR4 signalling; Activation of the AP-1 family of transcription factors; Amyotrophic lateral sclerosis (ALS); CD40/CD40L signaling; CDO in myogenesis; CXCR3-mediated signaling events; DSCAM interactions; Destabilization of mRNA by KSRP; ERK/MAPK targets; FAS (CD95) signaling pathway; Fc epsilon RI signaling pathway; GnRH signaling pathway; Hepatitis C; IL-4 signaling Pathway