Recombinant Human UCHL3, His-tagged

• Cat.No.: UCHL3-1518H
• Product Overview: Recombinant human UCHL3 protprot, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (1-230 aa).

Specification

• Cat. No.: UCHL3-1518H
• Description: Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as UCHL3, is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. UCHL3 play a role in the regulation of neuronal development and spermatogenesis and have been implicated in neurodegenerative diseases. UCHL3 protein is 54% identical to that of UCHL1.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT,10% glycerol.
• Molecular Weight: 28.3kDa (250aa), confirmed by MALDI-TOF
• Purity: > 95% by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Biological activity: Specific activity: >3,000 pmole/min/ug. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37°C.
• Activity Assay: 1. Prepare a 100ul of recombinant UCH-L3 protein with various concentrations (0.48ng, 0.9ng) in assay buffer and equilibrate to 37°C for 10 minutes. (Assay buffer: 50mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 0.1 mg/ml Ovalbumin, pH 8.0.) 2. Add 50ul of 1uM Ubiquitin-AMC. 3. Read at excitation wavelengths 355nm and emission 460nm for 5 minutes. - Ubiquitin-AMC- 96 Well Polystyrene Microplate, black - Fluorescent plate reader
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMDPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERARYLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGETSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: UCHL3 ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) [ Homo sapiens ]
• Official Symbol: UCHL3
• Synonyms: UCHL3; ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase); ubiquitin carboxyl-terminal hydrolase isozyme L3; ubiquitin thioesterase L3; ubiquitin thiolesterase; UCH-L3; EC 3.4.19.12
• Gene ID: 7347
• mRNA Refseq: NM_006002
• Protein Refseq: NP_005993
• MIM: 603090
• UniProt ID: P15374
• Chromosome Location: 13q22.2
• Function: cysteine-type peptidase activity; peptidase activity; ubiquitin thiolesterase activity; protein binding