"Vcam1" Related Products


Recombinant Mouse Vcam1, His-tagged

Cat. No.: Vcam1-4013M
Product Overview: A DNA sequence encoding the extracellular domain ( Met 1 - Glu 698 ) of mouse VCAM1 ( NP_035823.3 ) was fused with the a polyhistidine tag at the C-terminus.
Description: Mouse vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. Two isoforms of mouse VCAM1 have been identified, referred to as single-pass type I transmembrane (short) isoform 1 containing seven C2-type I g-like domains and GPI-anchored isoform 2. Mouse VCAM1 is expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.
Source: Mouse
Host: Human Cells
Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.
Purity: > 97 % as determined by SDS-PAGE
Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells / well are added to mouse VCAM1 coated plates ( 10 μg/mL with 100 μL/well ) , approximately 70% - 80% cells will adhere after 1 hour incubation at RT
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
Stability: Samples are stable for up to twelve months from date of receipt at -70μ
Predicted N terminal: Phe 25
Molecular Mass: The secreted recombinant mouse VCAM1 consists of 685 amino acids and has a predicted molecular mass of 75.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmVCAM1 is approximately 90-100 kDa due to glycosylation.
Storage: Store it under sterile conditions at -70μ. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Gene Name: Vcam1 vascular cell adhesion molecule 1 [ Mus musculus ]
Official Symbol: Vcam1
Synonyms: Vcam1; vascular cell adhesion molecule 1; CD106; Vcam-1
Gene ID: 22329
mRNA Refseq: NM_011693
Protein Refseq: NP_035823
UniProt ID: P29533
Chromosome Location: 3 G1; 3 50.8 cM
Pathway: Cell adhesion molecules (CAMs); Leukocyte transendothelial migration
Function: protein binding

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