Active Recombinant Human Vascular Endothelial Growth Factor A

• Cat.No.: VEGFA-583H
• Product Overview: Recombinant Human VEGF-121 produced in E.Coli is a double, non-glycosylated, polypeptide chain containing 121 amino acids and having a molecular mass of 28423 Dalton.

Specification

• Cat. No.: VEGFA-583H
• Description: Vascular endothelial growth factor A (VEGF-A) is a protein that in humans is encoded by the VEGFA gene.
• Species: Human
• Source: E.coli
• Form: Lyopohilized with no additives.
• Purity: Purity Greater than 95% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
• Bio-activity: The biological activity is determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 0.1-0.4 ng/ml.
• Physical Appearance: Sterile Filtered white lyophilized (freeze-dried) powder.
• Solubility: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized VEGF in sterile water at a concentration of 0.1 mg"s/ml, which can then be further diluted to other aqueous solutions.
• Purity Greater than 95% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
• Protein Content determined by: UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
• Endotoxin Level: Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
• Amino acid sequence: APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENCDKPR R
• Storage: Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Gene Information

• Gene Name: VEGFA vascular endothelial growth factor A [ Homo sapiens ]
• Official Symbol: VEGFA
• Synonyms: VPF; VEGF; VEGFA_HUMAN; Vascular permeability factor; VEGF-A; VEGFA; MGC70609; Vegf120; Vegf164; Vegf188; vascular endothelial growth factor A; MGC70609; Vascular endothelial growth factor A precursor; Vascular permeability factor
• Gene ID: 7422
• mRNA Refseq: NM_001025366
• Protein Refseq: NP_001020537
• MIM: 192240
• UniProt ID: P15692
• Chromosome Location: 6p12
• Pathway: Cytokine-cytokine receptor interaction; Focal adhesion; Pancreatic cancer; Renal cell carcinoma; VEGF signaling pathway; mTOR signaling pathway; Hemostasis; Signaling by VEGF.
• Function: cell surface binding ;extracellular matrix binding; growth factor activity; growth factor activity; heparin binding; platelet-derived growth factor receptor binding; protein binding; protein homodimerization activity; vascular endothelial growth factor receptor binding.