Recombinant Human VEGFB, His-tagged
Cat.No. : | VEGFB-554H |
Product Overview : | Recombinant VEGF-B protein was expressed in E.coli and purified by using conventional chromatography techniques (22-207 aa). |
- Specification
- Gene Information
- Related Products
Cat. No. : | VEGFB-554H |
Description : | VEGF-B, also known as Vascular endothelial growth factor-B, is one of 5 mammalian VEGF family members, the others being VEGF-A, VEGF-C, VEGF-D, and placental growth factor (PlGF). VEGF-B binds to one of the VEGF receptor tyrosine kinases, namely VEGF receptor-1(VEGFR1, Flt1), but not to VEGFR2 and VEGFR3. VEGF-B is homodimeric, heparin-binding glycoprotein involved in embryonic angiogenesis and vasculogenesis. |
Form : | Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol |
Molecular Weight : | 21.6 kDa (207aa), confirmed by MALDI-TOF. (molecular weight on SDS-PAGE will appear higher) |
Purity : | > 90% by SDS - PAGE |
Concentration : | 1 mg/ml (determined by Bradford assay) |
Endotoxin Level : | < 1.0 EU per 1μg of protein (determined by LAL method) |
Biological activity : | Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast. The ED50 for this effect is < 1ng/ml. |
Activity Assay : | 1. Cell line: NIH-3T3 (Mus mosculus/Mouse embryonic fibroblast ) 2. Maintenance Condition: RPMI 1640 containing 10% FBS 3. Assay medium: serum free RPMI 1640 4. Cell density: 2 x 104 cells/well (96 well plate, final volume 100ul) 5. Incubation time : 24 hr (after sample treatment) 6. Concentration range: 10pg/ml - 10ug/ml 7. Detection method : BrdU assay |
Sequences of amino acids : | MGSSHHHHHH SSGLVPRGSH MPVSQPDAPG HQRKVVSWID VYTRATCQPR EVVVPLTVEL MGTVAKQLVP SCVTVQRCGG CCPDDGLECV PTGQHQVRMQ ILMIRYPSSQ LGEMSLEEHS QCECRPKKKD SAVKPDRAAT PHHRPQPRSV PGWDSAPGAP SPADITHPTP APGPSAHAAP STTSALTPGP AAAAADAAAS SVAKGGA |
Storage : | Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles |
Gene Name : | VEGFB vascular endothelial growth factor B [ Homo sapiens ] |
Official Symbol : | VEGFB |
Synonyms : | VEGFB; VEGF-B; VEGFL; VRF; VEGF-related factor; vascular endothelial growth factor B; VEGF-related factor (VRF) |
Gene ID : | 7423 |
mRNA Refseq : | NM_003377 |
Protein Refseq : | NP_003368 |
MIM : | 601398 |
UniProt ID : | P49765 |
Chromosome Location : | 11q13 |
Pathway : | Cytokine-cytokine receptor interaction; Focal adhesion; Pancreatic cancer; Renal cell carcinoma; mTOR signaling pathway; Hemostasis; Signaling by VEGF |
Function : | growth factor activity; heparin binding; protein binding; vascular endothelial growth factor receptor binding |
Products Types
◆ Recombinant Protein | ||
VEGFB-0505H | Recombinant Human VEGFB Protein (M1-A207), His/Strep tagged | +Inquiry |
VEGFB-6170R | Recombinant Rat VEGFB Protein, His (Fc)-Avi-tagged | +Inquiry |
VEGFB-1554H | Recombinant Human VEGFB Protein (22-207 aa), His-tagged | +Inquiry |
VEGFB-565C | Recombinant Cattle VEGFB Protein, His-tagged | +Inquiry |
VEGFB-107H | Active Recombinant Human VEGFB Protein | +Inquiry |
◆ Lysates | ||
VEGFB-416HCL | Recombinant Human VEGFB 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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