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Recombinant Human APOA5, Flag-tagged

Cat.No. : APOA5-103H
Product Overview : Recombinant Human APOA5, fused with Flag-tag (11 extra AA) at C-terminal, was expressed in HEK293.
  • Specification
  • Gene Information
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Description : The protein encoded by this gene is an apolipoprotein that plays an important role in regulating the plasma triglyceride levels, a major risk factor for coronary artery disease. It is a component of high density lipoprotein and is highly similar to a rat protein that is upregulated in response to liver injury. Mutations in this gene have been associated with hypertriglyceridemia and hyperlipoproteinemia type 5. This gene is located proximal to the apolipoprotein gene cluster on chromosome 11q23. Alternatively spliced transcript variants encoding the same protein have been identified.
Source : HEK293
Species : Human
Tag : Flag
Form : Filtered (0.4 μm) and lyophilized in 0.5 mg/mL in 20mM TRIS, 50mM NaCl, pH 7.5
Molecular Mass : 40.1 kDa (calculated)
AA Sequence : RKGFWDYFSQ TSGDKGRVEQ IHQQKMAREP ATLKDSLEQD LNNMNKFLEK LRPLSGSEAP RLPQDPVGMR RQLQEELEEV KARLQPYMAE AHELVGWNLE GLRQQLKPYT MDLMEQVALR VQELQEQLRV VGEDTKAQLL GGVDEAWALL QGLQSRVVHH TGRFKELFHP YAESLVSGIG RHVQELHRSV APHAPASPAR LSRCVQVLSR KLTLKAKALH ARIQQNLDQL REELSRAFAG TGTEEGAGPD PQMLSEEVRQ RLQAFRQDTY LQIAAFTRAI DQETEEVQQQ LAPPPPGHSA FAPEFQQTDS GKVLSKLQAR LDDLWEDITH SLHDQGHSHL GDPAAADYKD DDDK
Endotoxin : <1.0>
Purity : Purity as determined by densitometric image analysis >70%
Applications : Cell culture and/or animal studies; ELISA; Western blotting
Quality Control Test : BCA to determine quantity of the protein.SDS PAGE to determine purity of the protein.LAL TEST to determine quantity of the endotoxin.
Notes : This product is intended for research use only.
Storage : Store lyophilized protein at –80°C. Lyophilized protein remains stable until the expiry date when stored at –80°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a week.
Reconstitution : Add deionized water to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Shipping : At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Gene Name : APOA5 apolipoprotein A-V [ Homo sapiens ]
Official Symbol : APOA5
Synonyms : APOA5; apolipoprotein A-V; APOA V; RAP3; apo-AV; apolipoprotein A5; regeneration-associated protein 3; APOAV; FLJ97995; MGC126836; MGC126838
Gene ID : 116519
mRNA Refseq : NM_001166598
Protein Refseq : NP_001160070
MIM : 606368
UniProt ID : Q6Q788
Chromosome Location : 11q23
Pathway : Chylomicron-mediated lipid transport; Fatty acid, triacylglycerol, and ketone body metabolism; PPAR signaling pathway
Function : enzyme activator activity; heparin binding; contributes_to heparin binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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