Active Recombinant Human GSTP1, His-tagged

• Cat.No.: GSTP1-1086H
• Product Overview: Human GSTP1 (NP_000843, 1 a.a. - 210 a.a.) full-length recombinant protein with His tag expressed in Escherichia coli.

Specification

• Cat. No.: GSTP1-1086H
• Description: GSTP1 is a glutathione S-transferase that belongs to the pi class. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide variety of electrophiles, including reactive products of lipid, protein, carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress.
• Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMPP YTVVYFPVRG RCAALRMLLA DQGQSWKEEV VTVETWQEGS LKASCLYGQL PKFQDGDLTL YQSNTILRHL GRTLGLYGKD QQEAALVDMV NDGVEDLRCK YISLIYTNYE AGKDDYVKAL PGQLKPFETL LSQNQGGKTF IVGDQISFAD YNLLDLLLIH EVLAPGCLDA FPLLSAYVGR LSARPKLKAF LASPEYVNLP INGNGKQ
• Theoretical MW (kDa): 27.6
• Form: Liquid
• Preparation Method: E. coli expression system
• Purity: > 95% by SDS-PAGE
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Activity: Specific activity is < 6.5 units/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25°C.
• Application: SDS-PAGE
• Storage Buffer: In 20 mM Tris-HCl, 1 mM EDTA, 0.1 mM PMSF, pH 7.0 (30% glycerol)
• Storage: Store at 4°C for 1-2 weeks. For long term storage store at -20°C or -80°C. Aliquot to avoid repeated freezing and thawing.
• Unitprot ID: P09211

Gene Information

• Gene Name: GSTP1 glutathione S-transferase pi 1 [ Homo sapiens ]
• Official Symbol: GSTP1
• Synonyms: GSTP1; glutathione S-transferase pi 1; PI; DFN7; GST3; FAEES3; deafness, X-linked 7; fatty acid ethyl ester synthase III; EC 2.5.1.18; 3` partial; GST class-pi; GSTP1-1; OTTHUMP00000174659; glutathione transferase
• Gene ID: 2950
• mRNA Refseq: NM_000852
• Protein Refseq: NP_000843
• MIM: 134660
• Chromosome Location: 11q13-qter
• Pathway: Drug metabolism - cytochrome P450; Glutathione metabolism; Metabolism of xenobiotics by cytochrome P450; Pathways in cancer; Prostate cancer; Biological oxidations
• Function: glutathione transferase activity; protein binding; transferase activity