Active Recombinant Human Thioredoxin Reductase 1

• Cat.No.: TXNRD1-1117H
• Product Overview: HumanTXNRD1 (161 a.a. - 647 a.a.) partial recombinant protein with His tagexpressed in E. coli.

Specification

• Cat. No.: TXNRD1-1117H
• Description: TXNRD1is an enzyme that in humans is encoded by the TXNRD1 gene. This gene encodesa member of the family of pyridine nucleotide oxidoreductases. This proteinreduces thioredoxins as well as other substrates, and plays a role inselenium metabolism and protection against oxidative stress. The functionalenzyme is thought to be a homodimer which uses FAD as a cofactor.
• Source: E. coli
• Species: Human
• Form: Liquid
• Molecular Weight: 55.7 kDa
• Purity: >95% by SDS-PAGE.
• Activity: Specificactivity is 0.48-0.6 units/mL, and was measured in a coupled assay with DTNBand NADPH. The amount of TNB generated by NADPH was measured in absorbance at412 nm.
• Buffer: In PBS, pH7.4 (10% glycerol).
• Quality ControlTesting: Loading 3 ugprotein in 15% SDS-PAGE.
• Purification: ConventionalChromatography.
• Concentration: 0.5 mg/mL
• Sequences of aminoacids: MGSSHHHHHH SSGLVPRGSH MYDYDLIIIG GGSGGLAAAK EAAQYGKKVMVLDFVTPTPL GTRWGLGGTC VNVGCIPKKL MHQAALLGQA LQDSRNYGWK VEETVKHDWD RMIEAVQNHI GSLNWGYRVALREKKVVYEN AYGQFIGPHR IKATNNKGKE KIYSAERFLI ATGERPRYLG IPGDKEYCIS SDDLFSLPYCPGKTLVVGAS YVALECAGFL AGIGLDVTVM VRSILLRGFD QDMANKIGEH MEEHGIKFIR QFVPIKVEQIEAGTPGRLRV VAQSTNSEEI IEGEYNTVML AIGRDACTRK IGLETVGVKI NEKTGKIPVT DEEQTNVPYIYAIGDILEDK VELTPVAIQA GRLLAQRLYA GSTVKCDYEN VPTTVFTPLE YGACGLSEEK AVEKFGEENIEVYHSYFWPL EWTIPSRDNN KCYAKIICNT KDNERVVGFH VLGPNAGEVT QGFAAALKCG LTKKQLDSTIGIHPVCAEVF TTLSVTKRSG ASILQAGC
• Storage: Storeat 4°C for 1-2 weeks. For long term storage store at -20°C or -80°C. Aliquotto avoid repeated freezing and thawing.

Gene Information

• Gene Name: TXNRD1 thioredoxinreductase 1 [ Homo sapiens ]
• Official Symbol: TXNRD1
• Synonyms: TXNRD1;thioredoxin reductase 1; TR; TR1; TXNR; TRXR1; GRIM-12; MGC9145; thioredoxinreductase 1, cytoplasmic; oxidoreductase; thioredoxin reductase TR1;thioredoxin reductase GRIM-12; KM-102-derived reductase-like factor; geneassociated with retinoic and IFN-induced mortality 12 protein; gene associatedwith retinoic and interferon-induced mortality 12 protein; EC 1.8.1.9; EC1.8.1; OTTHUMP00000232986; OTTHUMP00000232988; OTTHUMP00000232990;OTTHUMP00000232992; OTTHUMP00000232993; OTTHUMP00000232996;OTTHUMP00000232997; OTTHUMP00000232999
• Gene ID: 7296
• mRNA Refseq: NM_003330
• Protein Refseq: NP_003321
• MIM: 601112
• UniProt ID: Q16881
• Chromosome Location: 12q23-q24.1
• Pathway: Fatty acid triacylglycerol and ketone bodymetabolism; Metabolism; Metabolism of lipids and lipoproteins; Metabolism ofnucleotides; Oxidative Stress; PPARA Activates Gene Expression; Pyrimidinemetabolism; Regulation of Lipid Metabolism by Peroxisomeproliferator-activated receptor alpha (PPARalpha); Selenium Metabolism andSelenoproteins; Selenium Pathway; Selenocompound metabolism; Synthesis andinterconversion of nucleotide di- and triphosphates; selenium; thioredoxinpathway
• Function: NADP binding; electron carrier activity;flavin adenine dinucleotide binding; oxidoreductase activity; proteindisulfide oxidoreductase activity; thioredoxin-disulfide reductase activity