| Description : |
The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes an ubiquitously expressed, cytosolic form of TrxR, which functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing, primarily at the 5' end, results in transcript variants encoding same or different isoforms, including a glutaredoxin-containing isoform that is predominantly expressed in testis. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
54.6 kDa |
| AA Sequence : |
MNGPEDLPKSYDYDLIIIGGGSGGL AAAKEAAQYGKKVMVLDFVTPTPLG TRWGLGGTCVNVGCIPKKLMHQAAL LGQALQDSRNYGWKVEETVKHDWDR MIEAVQNHIGSLNWGYRVALREKKV VYENAYGQFIGPHRIKATNNKGKEK IYSAERFLIATGERPRYLGIPGDKE YCISSDDLFSLPYCPGKTLVVGASY VALECAGFLAGIGLDVTVMVRSILL RGFDQDMANKIGEHMEEHGIKFIRQ FVPIKVEQIEAGTPGRLRVVAQSTN SEEIIEGEYNTVMLAIGRDACTRKI GLETVGVKINEKTGKIPVTDEEQTN VPYIYAIGDILEDKVELTPVAIQAG RLLAQRLYAGSTVKCDYENVPTTVF TPLEYGACGLSEEKAVEKFGEENIE VYHSYFWPLEWTIPSRDNNKCYAKI ICNTKDNERVVGFHVLGPNAGEVTQ GFAAALKCGLTKKQLDSTIGIHPVC AEVFTTLSVTKRSGASILQAGCUGT RTRPLEQKLISEEDLAANDILDYKD DDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
