Active Recombinant Human CNTF protein, His-tagged

• Cat.No.: CNTF-566H
• Product Overview: Recombinant Human CNTF protein(Met1-Met200), fused with C-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-200 aa
• Form: Lyophilized from a 0.22 μm filtered solution of 20mM Tris, 150mM NaCl, pH 8.0.
• Bio-activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cell line. The ED50 for this effect is 13.5-54 ng/mL.
• Molecular Mass: 23.77 kDa
• Endotoxin: < 1 EU/μg of the protein by LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: CNTF ciliary neurotrophic factor [ Homo sapiens ]
• Official Symbol: CNTF
• Synonyms: CNTF; ciliary neurotrophic factor; HCNTF
• Gene ID: 1270
• mRNA Refseq: NM_000614
• Protein Refseq: NP_000605
• MIM: 118945
• UniProt ID: P26441