Recombinant Human Chloride Intracellular Channel 1, His-tagged

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Recombinant Human Chloride Intracellular Channel 1, His-tagged

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Cat. No. : CLIC1-2498H
Product Overview : Recombinant human CLIC1 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques.MW = 29.0 kDa (261aa).
Description : CLIC1 (Chloride intracellular channel 1), also referred to as NCC27, is a member of the highly conserved family of chloride ion channels that function in both soluble and integral membrane forms. This protein is a monomeric protein that contains a redox-active site similar to glutaredoxin; it functions as an anion-selective channel. It forms a dimer when oxidized and is then able to form chloride ion channels in bilayers and vesicles, whereas a reducing environment prevents this from occurring.
Source : E.coli.
Form : Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.1M NaCl.
Purity : > 90% by SDS – PAGE.
Concentration : 1.0 mg/ml (determined by Bradford assay).
Sequences Of Amino Acids : MGSSHHHHHH SSGLVPRGSH MAEEQPQVEL FVKAGSDGAK IGNCPFSQRL FMVLWLKGVT FNVTTVDTKR RTETVQKLCP GGQLPFLLYG TEVHTDTNKI EEFLEAVLCP PRYPKLAALN PESNTAGLDI FAKFSAYIKN SNPALNDNLE KGLLKALKVL DNYLTSPLPE EVDETSAEDE GVSQRKFLDG NELTLADCNL LPKLHIVQVV CKKYRGFTIP EAFRGVHRYL SNAYAREEFA STCPDDEEIE LAYEQVAKAL K.
Storage : can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
PDB rendering based on 1k0m. :

Gene Name : CLIC1 chloride intracellular channel 1 [ Homo sapiens ]
Synonyms : chloride intracellular channel 1; G6; NCC27; CLIC1; chloride intracellular channel protein 1; hRNCC; p64CLCP; RNCC protein; OTTHUMP00000029133; OTTHUMP00000029137; OTTHUMP00000174486; chloride channel ABP; nuclear chloride ion channel 27; nuclear chloride ion channel protein; regulatory nuclear chloride ion channel protein;Chloride channel ABP; Nuclear chloride ion channel 27; Regulatory nuclear chloride ion channel protein
Gene ID : 1192
mRNA Refseq : NM_001288
Protein Refseq : NP_001279
MIM : 602872
UniProt ID : O00299
Chromosome Location : 6p22.1-p21.2
Function : chloride channel activity; protein binding; voltage-gated chloride channel activity; voltage-gated ion channel activity

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