| Description : |
CCL20, also named LARC (Liver and Activation-regulated Chemokine), is encoded by CCL20 located on chromosome 2 in humans. The expression can be induced by LPS and some inflammatory cytokines, and it is at a high level in lymph nodes, PBL, fatal liver, fatal lung, appendix and so on. CCL20 has functions of chemotactic towards lymphocytes and dendritic cells. Additionally, it promotes the adhesion of memory CD4+ T cells and inhibits colony formation of bone marrow myeloid immature progenitors. CCL20 elicits it effects by binding with CCR6 receptor. |
| Source : |
E.coli |
| Species : |
Human |
| Form : |
Lyophilized from a 0.2μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl. |
| Bio-activity : |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-50 ng/ml. |
| Molecular Mass : |
Approximately 8.0 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids. |
| Protein length : |
70 |
| AA Sequence : |
ASNFDCCLGYTDRILHPKFIVGFTR QLANEGCDINAIIFHTKKKLSVCAN PKQTWVKYIVRLLSKKVKNM |
| Endotoxin : |
Less than 1 EU/μg of rHuMIP-3α/CCL20 as determined by LAL method. |
| Purity : |
>97% by SDS-PAGE and HPLC analysis. |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
