| Cat.No. : |
PIK3R1-4934H |
| Product Overview : |
Recombinant full-length human PI3K (p85alpha) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. |
| Description : |
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. |
| Source : |
Insect cells |
| Species : |
Human |
| Tag : |
His |
| Molecular Mass : |
~86 kDa |
| Purity : |
> 95% |
| Applications : |
Kinase Assay, Western Blot |
| Stability : |
One year at -70 centigrade from date of shipment. |
| Storage : |
Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
| Concentration : |
0.2 μg/μL |
| Storage Buffer : |
Recombinate protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0,1mM PMSF, 0,25mM DTT, 25% glycerol. |
| Purity : |
|
