Recombinant Human PIK3R1 Protein, His-tagged

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Recombinant Human PIK3R1 Protein, His-tagged

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Cat.No. : PIK3R1-4934H
Product Overview : Recombinant full-length human PI3K (p85alpha) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
Description : Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.
Source : Insect cells
Species : Human
Tag : His
Molecular Mass : ~86 kDa
Purity : > 95%
Applications : Kinase Assay, Western Blot
Stability : One year at -70 centigrade from date of shipment.
Storage : Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration : 0.2 μg/μL
Storage Buffer : Recombinate protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0,1mM PMSF, 0,25mM DTT, 25% glycerol.
Purity :

Gene Name : PIK3R1 phosphoinositide-3-kinase regulatory subunit 1 [ Homo sapiens (human) ]
Official Symbol : PIK3R1
Synonyms : p85; AGM7; GRB1; IMD36; p85-ALPHA; PIK3R1; phosphoinositide-3-kinase regulatory subunit 1; phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphatidylinositol 3-kinase-associated p-85 alpha; phosphoinositide-3-kinase regulatory subunit alpha; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); ptdIns-3-kinase regulatory subunit alpha
Gene ID : 5295
mRNA Refseq : NM_181523
Protein Refseq : NP_852664
MIM : 171833
UniProt ID : P27986

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