||Bone Morphogenetic protein-2 produced inE. coliis a homodimeric, non-glycosylated, Polypeptide chain containing 115 amino acids and having a molecular mass of 26.018 kDa.
||Bone morphogenetic protein 2 (BMP-2) is a protein that belongs to the TGF-β superfamily of proteins. It, like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. It is involved in the Hedgehog pathway, TGF-beta signaling pathway, and the Cytokine-cytokine receptor interaction. It is involved also in cardiac cell differentiation and epithelial to mesenchymal transition.
||>95% by SDS-PAGE and SEC-HPLC.
||Lyophilized from a concentrated (1mg/ml) sterile solution containing 10mM sodium citrate pH=3.5.
||BMP-2 is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of MC3T3-E1 cells is less than 50 ng/ml, corresponding to a specific activity of 2.0 x 104IU/mg.
||Less than 0.1ng/µg (1IEU/µg) of Bone Morphogenetic protein-2 .
||It is recommended to reconstitute the lyophilized rHuBMP2 in sterile 20mM Acetic acid not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
||Lyophilized BMP2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BMP2 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freeze-thaw cycles.