Recombinant Human Cell Division Cycle 25 Homolog C (S. pombe), GST-tagged

• Cat.No.: CDC25C-1576H
• Product Overview: Full-length recombinant human CDC25C was expressed in Sf9 insect cells using an N-terminal GST tag.

Specification

• Cat. No.: CDC25C-1576H
• Description: CDC25C (also known as cell division cycle 25 homolog C) is a member of the CDC25 phosphatase family. CDC25C is highly conserved during evolution and it plays a key role in the regulation of cell division. CDC25C activates the partially purified p34 (cdc2)/cyclin B complex and directs the dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. CDC25C also suppresses p53-induced growth arrest. The regulation of CDC25B phosphorylation by p38 is a critical event for initiating the G2/M checkpoint after ultraviolet radiation.
• Applications: Phosphatase Assay; Western Blot
• Molecular Weight: 84 kDa
• Expression System: Sf9 insect cells using baculovirus
• Species: Human
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
• Specific Activity: 22 nmol/min/mg
• Purity: > 70 %
• Concentration: 0.1 ug/ul
• Sequences: Full-length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Activation of ATR in response to replication stress; Cell Cycle Checkpoints; Cell Cycle, Mitotic; Cell cycle; Cyclin A/B1 associated events during G2/M transition; Cyclin B2 mediated events; G2/M Checkpoints; G2/M DNA damage checkpoint; G2/M Transition; Monoamine Transport; Oocyte meiosis; PLK1 signaling events; PLK3 signaling events; Polo-like kinase mediated events; Progesterone-mediated oocyte maturation

Gene Information

• Gene Name: CDC25C cell division cycle 25 homolog C (S. pombe) [ Homo sapiens ]
• Official Symbol: CDC25C
• Synonyms: CDC25C; cell division cycle 25 homolog C (S. pombe); M-phase inducer phosphatase 3; OTTHUMP00000159490; cell division cycle 25C; dual specificity phosphatase CDC25C; mitosis inducer CDC25; phosphotyrosine phosphatase; CDC25; EC 3.1.3.48
• Gene ID: 995
• mRNA Refseq: NM_001790
• Protein Refseq: NP_001781
• MIM: 157680
• UniProt ID: P30307
• Chromosome Location: 5q31
• Function: WW domain binding; hydrolase activity; phosphoprotein phosphatase activity; protein binding; protein tyrosine phosphatase activity