Recombinant Human E1A Binding Protein P300, GST-tagged

• Cat.No.: EP300-1585H
• Product Overview: Recombinant human EP300 (1283-1673) was expressed by baculovirus in Sf9 cells using a N-terminal GST tag.

Specification

• Cat. No.: EP300-1585H
• Description: EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. P300 is related by sequence to CBP and like CBP can stimulate transcription through activation of CREB. The EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. EP300 is a components of DRAF1 (double-stranded RNA-activated factor-1), a positive regulator of interferon-stimulated gene transcription that functions as a direct response to viral infection.
• Applications: Western Blot
• Molecular Weight: 74 kDa
• Expression System: Sf9 insect cells using baculovirus
• Species: Human
• Form: Recombinant protein stored in 50 mM Tris-HCl, ph 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
• Purity: > 70 %
• Concentration: 0.2 ug/ul
• Sequences: 1283-1673
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: ATF-2 transcription factor network; Adherens junction; Androgen Receptor Signaling Pathway; C-MYB transcription factor network; Cell cycle; Delta-Notch Signaling Pathway; Diabetes pathways; Direct p53 effectors; E2F transcription factor network; Energy Metabolism; FOXA1 transcription factor network

Gene Information

• Gene Name: EP300 E1A binding protein p300 [ Homo sapiens ]
• Official Symbol: EP300
• Synonyms: EP300; E1A binding protein p300; p300; KAT3B; RSTS2; histone acetyltransferase p300; p300 HAT; E1A-binding protein, 300Kd; E1A-associated protein p300; OTTHUMP00000198754
• Gene ID: 2033
• mRNA Refseq: NM_001429
• Protein Refseq: NP_001420
• MIM: 602700
• UniProt ID: Q09472
• Chromosome Location: 22q13.2
• Function: DNA binding; NF-kappaB binding; RNA polymerase II activating transcription factor binding; SMAD binding; acetyltransferase activity; activating transcription factor binding; acyltransferase activity; bHLH transcription factor binding; glucocorticoid receptor binding; histone acetyltransferase activity; metal ion binding; peroxisome proliferator activated receptor binding; transcription coactivator activity; contributes_to transcription coactivator activity; zinc ion binding