Recombinant Human Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4, GST-tagged

• Cat.No.: MAP4K4-1042H
• Product Overview: Recombinant human MAP4K4 (1-328) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Cat. No.: MAP4K4-1042H
• Description: MAP4K4 is the mitogen-activated protein kinase kinase kinase kinase 4(MAP4K4) and a member of the serine/threonine protein kinase family. MAP4K4 has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by MAP4K4 can be inhibited by dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggest that this kinase functions through the MAP3K7-MAP2K4-MAP2K7 kinase cascade and mediates TNF-α signaling. MAP4K4-dependent signaling inhibits PPARγ-responsive gene expression, adipogenesis, and insulin-stimulated glucose transport.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 64 kDa
• Expression System: Sf9 insect cells using baculovirus.
• Species: Human
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
• Specific Activity: 54 nmol/min/mg
• Purity: > 95 %
• Concentration: 0.1 ug/ul
• Sequences: 1-328
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Ceramide signaling pathway; Direct p53 effectors; EPHB forward signaling; Insulin Signaling; MAPK signaling pathway; TNF receptor signaling pathway

Gene Information

• Gene Name: MAP4K4 mitogen-activated protein kinase kinase kinase kinase 4 [ Homo sapiens ]
• Official Symbol: MAP4K4
• Synonyms: MAP4K4; mitogen-activated protein kinase kinase kinase kinase 4; HGK; NIK; FLH21957; FLJ10410; FLJ20373; FLJ90111; KIAA0687; MEKKK 4; MEK kinase kinase 4; nck-interacting kinase; HPK/GCK-like kinase HGK; MAPK/ERK kinase kinase kinase 4; hepatocyte progenitor kinase-like/germinal center kinase-like kinase; EC=2.7.11.1; OTTHUMP00000207902; OTTHUMP00000207903; OTTHUMP00000207971; OTTHUMP00000207972; OTTHUMP00- 000207973; OTTHUMP00000207974
• Gene ID: 9448
• mRNA Refseq: NM_004834
• Protein Refseq: NP_004825
• MIM: 604666
• UniProt ID: O95819
• Chromosome Location: 2q11.2-q12
• Function: ATP binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; small GTPase regulator activity