"PIK3CA" Related Products


Recombinant Human Phosphoinositide-3-kinase, Catalytic, Alpha Polypeptide, His-tagged

Cat. No. : PIK3CA-1078H
Product Overview : Recombinant full-length human p110 alpha (E545K) mutant subunit and human p85 alpha wild-type subunit were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.
Description : The PIK3CA comprises of a 110 kDa catalytic subunit and a 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. The p110α catalytic subunit (PIK3CA) is frequently mutated or amplified in a variety of cancers including ovarian and colon and this protein is one of the PIK3CA mutants. PIK3CA gene copy number is increased in over 30% of ovarian cancers and this leads to increased PI3-kinase activity. Furthermore, the activity of p110α is essential for vascular development and inactivation of p110α leads to severe defects in angiogenic sprouting and vascular remodeling.
Applications : Kinase Assay; Western Blot
Molecular Weight : 111 kDa (p110α), 86 kDa (p85α)
Expression System : Sf9 insect cells using baculovirus
Species : Human
Form : Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.
Specific Activity : 50 nmol/min/mg
Purity : > 70 %
Concentration : 0.1 ug/ul
Sequences : Full-length
Storage : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Pathways : 3-phosphoinositide biosynthesis; Acute myeloid leukemia; Acute myeloid leukemia; Adaptive Immunity Signaling; B cell receptor signaling pathway; Bacterial invasion of epithelial cells; CDC42 signaling events; Downstream signaling of activated FGFR; EPHA2 forward signaling; FGF signaling pathway; HTLV-I infection; IL23-mediated signaling events; Inositol phosphate metabolism; Jak-STAT signaling pathway; Non-small cell lung cancer
PI3 Kinase 110 alphabound to the inhibitor PIK-93 (yellow). :

Gene Name : PIK3CA phosphoinositide-3-kinase, catalytic, alpha polypeptide [ Homo sapiens ]
Official Symbol : PIK3CA
Synonyms : PIK3CA; phosphoinositide-3-kinase, catalytic, alpha polypeptide; PI3K; MGC142161; MGC142163; p110-alpha; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; PI3K-alpha; OTTHUMP00000211945; PtdIns-3-kinase p110; PI3-kinase subunit alpha; PI3-kinase p110 subunit alpha; ptdIns-3-kinase subunit alpha; ptdIns-3-kinase subunit p110-alpha; phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha; phosphatidylinositol 3-kinase, catalytic, alpha polypeptide; phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform
Gene ID : 5290
mRNA Refseq : NM_006218
Protein Refseq : NP_006209
MIM : 171834
UniProt ID : P42336
Chromosome Location : 3q26.3
Function : 1-phosphatidylinositol-3-kinase activity; ATP binding; inositol or phosphatidylinositol kinase activity; insulin receptor substrate binding; nucleotide binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; phosphotransferase activity, alcohol group as acceptor; protein binding; protein serine/threonine kinase activity

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