| Cat. No. : |
PRMT5-1618H |
| Product Overview : |
Recombinant full-length human PRMT5 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. |
| Description : |
PRMT5 is a protein arginine methyltransferase and part of a 20S methyltransferase complex that modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. MEP50 associates with PRMT5 in the 20S arginine methyltransferase complex in HeLa cells. Antibodies directed against MEP50 can reduce the arginine methyltransferase activity of the immunopurified complex toward Sm substrates. PRMT5-mediated transcriptional repression of reporter gene is dependent on MEP50. |
| Applications : |
Western Blot |
| Molecular Weight : |
96 kDa |
| Expression System : |
Sf9 insect cells using baculovirus |
| Species : |
Human |
| Form : |
Recombinant protein stored in 50 mM Tris-HCL, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol, 0.1 mM EDTA. |
| Purity : |
> 95 % |
| Concentration : |
0.1 ug/ul |
| Storage : |
Store product at -70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.