Recombinant Human Sirtuin 7, His-tagged

• Cat.No.: SIRT7-4884H
• Product Overview: Full-length recombinant human SIRT7 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Specification

• Cat. No.: SIRT7-4884H
• Description: SIRT7 is a member of the class IV of sirtuin family of proteins which are homologs to the yeast Sir2 protein and play a role in cell differentiation, proliferation, apoptosis, metabolism, and senescence. SIRT7 associates with active rRNA genes and histones. Overexpression of SIRT7 increases pol I-mediated transcription whereas knockdown of SIRT7 or inhibition of its catalytic activity results in decreased association of pol I with rDNA and reduced pol I transcription.
• Applications: SIRT Assay
• Molecular Weight: 46 kDa
• Expression System: Sf9 insect cells using baculovirus
• Species: Human
• Form: Recombinant protein stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.
• Specific Activity: 140 RLU/min/mg
• Purity: > 95 %
• Concentration: 0.1 ug/ul
• Sequences: Full length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Signaling events mediated by HDAC Class I; Signaling events mediated by HDAC Class III

Gene Information

• Gene Name: SIRT7 sirtuin 7 [ Homo sapiens ]
• Official Symbol: SIRT7
• Synonyms: SIRT7; sirtuin 7; SIR2L7; MGC126840; MGC126842; NAD-dependent deacetylase sirtuin-7; sirtuin type 7; SIR2-like protein 7; sir2-related protein type 7; silent mating type information regulation 2, S.cerevisiae, homolog 7; EC 3.5.1.-; sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae); sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 7
• Gene ID: 51547
• mRNA Refseq: NM_016538
• Protein Refseq: NP_057622
• MIM: 606212
• UniProt ID: Q9NRC8
• Chromosome Location: 17q25.3
• Function: NAD binding; hydrolase activity; metal ion binding; protein binding; zinc ion binding