||Recombinant human RPIA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
||RPIA (Ribose-5-phosphate isomerase) belongs to the ribose 5-phosphate isomerase family. RPIA is an enzyme that catalyzes the conversion between ribose-5-phosphate (R5P) and ribulose-5-phosphate (Ru5P). RPI exists as two distinct proteins forms, termed RPIA and RPIB. RPIA plays an essential role in the metabolism of plants and animals, as it is involved in the Calvin cycle which takes place in plants, and the pentose phosphate pathway which takes place in plants as well as animals.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT,40% glycerol, 200mM NaCl, 2mM EDTA, 0.2mM PMSF.
||35.4kDa (331aa), confirmed by MALDI-TOF.
||>90% by SDS-PAGE
||0.25 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MQRPGPFSTL YGRVLAPLPG RAGGAASGGG GNSWDLPGSH VRLPGRAQSG TRGGAGNTST SCGDSNSICP APSTMSKAEE AKKLAGRAAV ENHVRNNQVL GIGSGSTIVH AVQRIAERVK QENLNLVCIP TSFQARQLIL QYGLTLSDLD RHPEIDLAID GADEVDADLN LIKGGGGCLT QEKIVAGYAS RFIVIADFRK DSKNLGDQWH KGIPIEVIPM AYVPVSRAVS QKFGGVVELR MAVNKAGPVV TDNGNFILDW KFDRVHKWSE VNTAIKMIPG VVDTGLFINM AERVYFGMQD GSVNMREKPF C
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||Metabolic pathways; Metabolism of carbohydrates; Pentose Phosphate Pathway