Active Recombinant Human IGFBP7 protein(Met1-Leu282), hFc-tagged

• Cat.No.: IGFBP7-5165H
• Product Overview: Recombinant Human IGFBP7 (Q16270) (Met 1-Leu 282) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: C-hFc
• Protein length: Met1-Leu282
• Form: Lyophilized from sterile 25mM Sodium CitRate, 200mM NaCl, 0.02%tween 20, 4% Trehalose, 10%glycerol, pH 6.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Human CD93 (His Tag) at 2 μg/ml (100 μl/well) can bind Human IGFBP7 (hFc Tag). The EC50 is 8-60 ng/ml.
• Molecular Mass: The secreted recombinant human IGFBP7/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 497 amino acids and has a predicted molecular mass of 53.5 kDa. The apparent molecular mass of rh IGFBP7/Fc monomer is approximately 65-70 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IGFBP7 insulin-like growth factor binding protein 7 [ Homo sapiens ]
• Official Symbol: IGFBP7
• Synonyms: IGFBP7; insulin-like growth factor binding protein 7; insulin-like growth factor-binding protein 7; FSTL2; IGFBP 7; MAC25; PSF; IGFBP-rP1; angiomodulin; IGF-binding protein 7; PGI2-stimulating factor; tumor-derived adhesion factor; prostacyclin-stimulating factor; AGM; TAF; IBP-7; IGFBP-7; RAMSVPS; IGFBP-7v; IGFBPRP1
• Gene ID: 3490
• mRNA Refseq: NM_001253835
• Protein Refseq: NP_001240764
• MIM: 602867
• UniProt ID: Q16270