||Recombinant Human Interferon alpha-2b produced inE. coliis a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19269 Dalton. Pegylated IFN alpha-2b is manufactured by attaching a 40KD NHS-mPEG to the primary amino acid of IFN alpha-2b.
||At least 23 different variants of IFN-alpha are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.
||>98% as determined by SDS-PAGE , RP-HPLC and FPLC.
||Lyophilized from a (0.5mg/ml) solution in containing 8.8mg sodium chloride, 0.4mg sodium acetate and 1.0mg acetate acid buffer.
||The specific activity as determined in a viral resistance assay using VSV-WISH cells was found to be 1.0 x 106IU/mg.
||Less than 0.1 ng/µg (IEU/µg) of PEG-IFN alpha-2b.
||It is recommended to reconstitute the lyophilized PEG-IFN alpha-2b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
||Lyophilized PEG-IFN alpha-2b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PEG-IFN alpha-2b should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freeze-thaw cycles.