Recombinant Human Ubiquitin Conjugating Enzyme 9, His6-Tagged


Home / Products / Recombinant Proteins / Recombinant Human Ubiquitin Conjugating Enzyme 9, His6-Tagged

Recombinant Human Ubiquitin Conjugating Enzyme 9, His6-Tagged

UBE2I Related Products

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

Cat. No. : UBE2I -03H
Product Overview : Recombinant Human Ubiquitin Conjugating Enzyme 9 produced inE.coliis a single non-glycosylated polypeptide chain containing 171 amino acids and having a molecular mass of approximately 19.5 kDa.
Description : Human Ubquitin Conjugating Enzyme 9 (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumolyation, and may play a role in substrate recognition. Ubc9 can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, IκBα, and PML without the requirement of an E3 ligase.
Purity : >95% by SDS-PAGE and HPLC analyses.
Formulation : Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in 1×PBS, 1mM DTT, pH 7.5.
Endotoxin : Less than 1EU/μg of rHuUbc9 as determined by LAL method.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20oC. Further dilutions should be made in appropriate buffered solutions.
Storage : This lyophilized preparation is stable at 2-8oC, but should be kept at -20oC for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8oC. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20oC to -70oC. Avoid repeated freeze/thaw cycles.
PDBrendering based on 1a3s. Available structures:1a3s, 1kps, 1u9a, 1u9b, 1z5s2grn, 2gro, 2grp, 2grq, 2grr, 2o25, 2pe6 :

Gene Name : UBE2I
Synonyms : P18; UBC9; C358B7.1; ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast); ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9); UBC9_HUMAN; SUMO-conjugating enzyme UBC9; EC6.3.2.-; SUMO-protein ligase; Ubiquitin-protein ligase I; Ubiquitin carrier protein I; Ubiquitin carrier protein 9; UBE2I;UBCE9; Ubiquitin-protein ligase I; ubiquitin carrier protein; ubiquitin conjugating enzyme 9; ubiquitin-conjugating enzyme E2I; ubiquitin-conjugating enzyme UbcE2A; ubiquitin-like protein SUMO-1 conjugating enzyme; ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
Gene ID : 7329
mRNA Refseq : NM_003345
Protein Refseq : NP_003336
MIM : 601661
UniProt ID : P63279
Chromosome Location : 16p13.3
Function : HLH domain binding;ligase activity;protein binding; small conjugating protein ligase activity.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry

  • Note: There will be extra charge for optional service!

Optional Service: Optional requirements on this protein

Other Requirements:

Apply For A Coupon

$50 OFF Your First Purchase

Apply For a Coupon

Enter your email here to subscribe.

creative biomart inc.

Easy access to products and services you need from our library via powerful searching tools.

Follow Us

Copyright © 2021 Creative BioMart. All Rights Reserved. Terms and Conditions | Privacy Policy