Recombinant Rat Fgf2 protein
Cat.No. : | Fgf2-40R |
Product Overview : | Recombinant Rat Fgf2 protein was expressed in Escherichia coli. |
- Specification
- Gene Information
- Related Products
Description : | Rat bFGF, encoded by the FGF2 gene, is a member of the fibroblast growth factor (FGF) family. Fibroblast growth factor was found in pituitary extracts in 1973 and then tested in a bioassay that caused fibroblasts to proliferate. After further fractionating the extract using acidic and basic pH, two different forms have isolated that named "acidic fibroblast growth factor" (FGF-1) and "basic fibroblast growth factor" (FGF-2). Rat bFGF shares 96 % amino acid sequence identity with human bFGF, and 98 % amino acid sequence identity with mouse. Affinity between bFGF and its receptors can be increased by heparin or heparan sulfate proteoglycan. bFGF plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. bFGF are also involved in a variety of biological processes, including embryonic development , morphogenesis, tissue repair, tumor growth and invasion. Additionally, bFGF is frequently used for a critical component of cell culture medium, e.g., human embryonic stem cell culture medium, serum-free culture systems. |
Source : | E.coli |
Species : | Rat |
Form : | Lyophilized from a 0.2μm filtered solution in PBS, pH 7.4. |
Bio-activity : | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 10⁶ IU/mg. |
Molecular Mass : | Approximately 16.4 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids. |
Protein length : | 146 |
AA Sequence : | MPALPEDGGGAFPPGHFKDPKRLYC KNGGFFLRIHPDGRVDGVREKSDPH VKLQLQAEERGVVSIKGVCANRYLA MKEDGRLLASKCVTEECFFFERLES NNYNTYRSRKYSSWYVALKRTGQYK LGSKTGPGQKAILFLPMSAKS |
Endotoxin : | Less than 1 EU/µg of rRtbFGF as determined by LAL method. |
Purity : | >98% by SDS-PAGE and HPLC analysis. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
Gene Name : | Fgf2 |
Official Symbol : | Fgf2 |
Synonyms : | FGF-2, HBGF-2 |
Gene ID : | 54250 |
mRNA Refseq : | NM_019305 |
Protein Refseq : | NP_062178 |
UniProt ID : | P13109 |
Products Types
◆ Recombinant Protein | ||
Fgf2-398F | Active Recombinant Mouse Fgf2 Protein (146 aa) | +Inquiry |
FGF2-16H | Active Recombinant Human FGF2 Protein, Animal Free | +Inquiry |
FGF2-401F | Active Recombinant Human FGF2 Protein (146 aa) | +Inquiry |
FGF2-001H | Recombinant Human FGF2 Protein, His-tagged | +Inquiry |
FGF2-4387R | Recombinant Rabbit FGF2 Protein | +Inquiry |
◆ Lysates | ||
FGF2-638HCL | Recombinant Human FGF2 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (6)
Ask a questionFGF2 promotes cell migration and angiogenesis and regulates bone homeostatic balance.
FGF2 is widely expressed in the nervous system.
FGF2 is mainly expressed in tissues such as mammary glands and ovaries of sheep.
FGF1 has a high degree of homology with FGF2.
FGF2 is involved in diseases such as cardiovascular disease, breast disease and bone homeostasis.
FGF2 negatively regulates bone homeostasis.
Customer Reviews (2)
Write a reviewThe technical expert communicated with me in time to understand the progress of my experiment and gave me suggestions for improvement.
The purchased protein is suitable for WB experiments and I am satisfied with the results.
Ask a Question for All Fgf2 Products
Required fields are marked with *
My Review for All Fgf2 Products
Required fields are marked with *
Inquiry Basket