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Recombinant Human CLU, Flag-tagged

Cat.No.: CLU-106H
Product Overview: The Clusterin Human contains a total 438 amino acids and a calculated molecular mass of 51.27kDa (calculated). The AA sequence (AA 1-427) is identical to Swiss-Prot-P10909 (AA 23-449, secreted Human Clusterin). C-terminal Flag-tag 11 extra AA (underlined).
Description: The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.
Source: 293 cell line (Human embryonic kidney)
Species: Human
Tag: Flag
Form: Filtered, White, Lyophilized powder. Filtered (0.4 micron) and lyophilized PBS, pH 7.5.
Molecular Mass: 51.27kDa
AA Sequence: DQTVSDNELQ EMSNQGSKYV NKEIQNAVNG VKQIKTLIEK TNEERKTLLS NLEEAKKKKE DALNETRESE TKLKELPGVC NETMMALWEE CKPCLKQTCM KFYARVCRSGS GLVGRQLEE FLNQSSPFYF WMNGDRIDSL LENDRQQTHM LDVMQDHFSRA SSIIDELFQ DRFFTREPQD TYHYLPFSLP HRRPHFFFPK SRIVRSLMPF SPYEPLNFHA MFQPFLEMIH EAQQAMDIHF HSPAFQHPPT EFIREGDDDR TVCREIRHNS TGCLRMKDQC DKCREILSVD CSTNNPSQAKLRRELDESLQ VAERLTRKYN ELLKSYQWKM LNTSSLLEQL NEQFNWVSRL ANLTQGEDQYYLRVTTVASH TSDSDVPSGV TEVVVKLFDS DPITVTVPVE VSRKNPKFME TVAEKALQEY RKKHREEAAA DYKDDDDK.
Purity: Greater than 95% as determined by SDS PAGE.
Storage: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product not sterile! Please filter the product by an appropriate sterile filter before using it in cell culture.
Gene Name: CLU clusterin [ Homo sapiens (human) ]
Official Symbol: CLU
Synonyms: CLU; CLI; AAG4; APOJ; CLU1; CLU2; KUB1; SGP2; APO-J; SGP-2; SP-40; TRPM2; TRPM-2; NA1/NA2; clusterin; apolipoprotein J; ku70-binding protein 1; sulfated glycoprotein 2; aging-associated protein 4; complement lysis inhibitor; complement cytolysis inhibitor; complement-associated protein SP-40,40; testosterone-repressed prostate message 2
Gene ID: 1191
mRNA Refseq: NM_001831
Protein Refseq: NP_001822
MIM: 185430
UniProt ID: P10909
Chromosome Location: 8p21-p12
Pathway: Platelet activation; Platelet degranulation; Response to elevated platelet cytosolic Ca2+
Function: NOT ATPase activity; misfolded protein binding; ubiquitin protein ligase binding

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