||A DNA sequence encoding Human TSLP was expressed.
||Thymic stromal lymphopoietin (TSLP) is an IL-7-like epithelial cell-derived cytokine that supports differentiation and long-term growth of B cells. It is expressed in the heart, liver, testis and prostate. TSLP overlaps biological activities with IL-7 and binds with the heterodimeric receptor complex consisting of the IL-7R alpha chain (IL-7Rα) and the TSLP-specific chain (TSLPR). Like IL-7, TSLP induces phosphorylation of STAT3 and STAT5 but uses kinases other than the JAKs for activation. It activates DCs primed na?ve T cells to produce the proallergic cytokines (IL-4, IL-5, IL-13, TNFα) while down-regulating IL-10 and IFN-γ suggesting a role in initiating allergic inflammation. TSLP prohibits apoptosis and stimulates growth of the human acute myeloid leukemia (AML)-derived cell line MUTZ3. It induces the release of T cell-attracting chemokines TARC and MDC from monocytes and activates CD11c(+) dendritic cells.
||This recombinant protein was 0.2 μm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
||The predicted molecular weight of Recombinant Human TSLP is Mr 15 kDa.
||myd ftncdfekik aaylstiskd litymsgtks tefnntvscs nrphclteiq sltfnptagc aslakemfam ktkaalaiwc pgysetqina tqamkkrrkr kvttnkcleq vsqlqglwrr fnrpllkqq
||<1.0 eu/μg as determined by the lal>1.0>
||>95% by SDS-PAGE and analyzed by silver stain.
||The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.