Recombinant Human POU5F1, His-tagged

• Cat.No.: POU5F1-8470H
• Product Overview: Recombinant Human POU5F1, fused with His tag, was expressed in E. coli and purified by affinity chromatography in combination with FPLC columns.

Specification

• Description: This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development. especially during early embryogenesis. and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing"s sarcoma gene. t(6.22)(p21.q12). has been linked to tumor formation. Alternative splicing. as well as usage of alternative translation initiation codons. results in multiple isoforms. one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1. 3. 8. 10. and 12.
• Source: E. coli
• Species: Human
• Tag: His
• Form: 20 mM Tris-Cl, pH 7.9, 20% Glycerol. 100 mM KCl. 1 mM DTT and 0.2 mM EDTA
• Molecular Mass: 38.6 kDa
• Endotoxin: Less than 1.0 EU/μg by LAL method
• Purity: >95% as determined by SDS-PAGE
• Storage: -80 °C
• Shipping: Dry Ice

Gene Information

• Gene Name: POU5F1 POU class 5 homeobox 1 [ Homo sapiens ]
• Official Symbol: POU5F1
• Synonyms: POU5F1; POU class 5 homeobox 1; OTF3, POU domain class 5, transcription factor 1; POU domain, class 5, transcription factor 1; MGC22487; OCT3; Oct4; octamer-binding protein 3; octamer-binding protein 4; POU domain transcription factor OCT4; octamer-binding transcription factor 3; octamer-binding transcription factor-3; POU-type homeodomain-containing DNA-binding protein; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
• Gene ID: 5460
• mRNA Refseq: NM_001173531
• Protein Refseq: NP_001167002
• MIM: 164177
• UniProt ID: Q01860
• Chromosome Location: 6p21.33
• Pathway: HIF-2-alpha transcription factor network, organism-specific biosystem; Wnt Signaling Pathway and Pluripotency, organism-specific biosystem
• Function: DNA binding; miRNA binding; protein binding; sequence-specific DNA binding; sequence-specific DNA binding RNA polymerase II transcription factor activity; sequence-specific DNA binding transcription factor activity; transcription factor binding; transcription regulatory region DNA binding; ubiquitin protein ligase binding