Active Recombinant Human Bone Morphogenetic Protein 5
Cat.No. : | BMP5-27H |
Product Overview : | Recombinant Human BMP5 (Ala317His454) was expressed in CHO Cells. |
- Specification
- Gene Information
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Cat. No. : | BMP5-27H |
Description : | This gene encodes a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. These proteins are synthesized as prepropeptides, cleaved, and then processed into dimeric proteins. This protein may act as an important signaling molecule within the trabecular meshwork and optic nerve head, and may play a potential role in glaucoma pathogenesis. This gene is differentially regulated during the formation of various tumors. |
Source : | CHO Cells |
Molecular Mass : | This protein has a calculated molecular mass of 15.6 kDa, migrates as an approximately 18-23 kDa band in SDS-PAGE under reducing conditions as predicted. |
Predicted N terminal : | Ala317 |
Formulation : | Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein. |
Purity : | >95%, by SDSPAGE under reducing conditions and visualized by silver stain. |
Activity : | Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is typically 0.2-1.2 µg/mL. |
EndotoxinLevel : | <0.01 EU per 1 μg of the protein by the LAL method. |
Reconstitution : | Reconstitute at 100 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin. |
Storage : | 12 months from date of receipt, 20 to 70 °C as supplied. Avoid repeated freezethaw cycles. |
OfficialSymbol : | BMP5 |
Gene Name : | BMP5 bone morphogenetic protein 5 [ Homo sapiens ] |
Synonyms : | BMP5; bone morphogenetic protein 5; MGC34244; BMP-5; OTTHUMP00000016650 |
Gene ID : | 653 |
mRNA Refseq : | NM_021073 |
Protein Refseq : | NP_066551 |
MIM : | 112265 |
UniProt ID : | P22003 |
Chromosome Location : | 6p12.1 |
Pathway : | TGF-beta signaling pathway |
Function : | BMP receptor binding; cytokine activity; growth factor activity |
Products Types
◆ Recombinant Protein | ||
BMP5-280H | Active Recombinant Human BMP5 Protein (Val341-His454), C-His tagged, Animal-free, Carrier-free | +Inquiry |
Bmp5-260M | Recombinant Mouse Bmp5 Protein, His-tagged | +Inquiry |
BMP5-014H | Active Recombinant Human BMP5 Protein | +Inquiry |
BMP5-4365H | Recombinant Human BMP5 Protein, His (Fc)-Avi-tagged | +Inquiry |
BMP5-15H | Recombinant Active Human BMP5 Protein, His-tagged(C-ter) | +Inquiry |
◆ Lysates | ||
BMP5-3074HCL | Recombinant Human BMP5 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionBMP5, a member of the bone morphogenetic protein family, functions by binding to cell surface receptors and activating the Smad signaling pathway. This leads to the phosphorylation and nuclear localization of transcription factors, thereby regulating gene expression. BMP5 plays a crucial role in cellular proliferation, differentiation, and fate determination. Its signaling mechanism involves various molecular regulations, including the involvement of co-receptors, signal transduction mediators, and downstream effectors.
BMP5 regulates the expression of various downstream target genes involved in multiple cellular processes. These include genes encoding transcription factors, extracellular matrix components, growth factors, and signaling molecules. Examples of BMP5 target genes include Runx2, Osterix, Sox9, Noggin, and Gremlin. Through the activation of these target genes, BMP5 influences cell fate determination, osteoblast differentiation, chondrogenesis, and tissue homeostasis.
BMP5 plays a critical role in skeletal development and bone formation. It promotes the commitment of mesenchymal stem cells to the osteoblast lineage and stimulates their differentiation into mature osteoblasts. BMP5 also regulates the proliferation and survival of osteoblasts, as well as the synthesis and mineralization of the extracellular matrix. Additionally, BMP5 interacts with other BMP family members and signaling molecules to coordinate the complex processes of skeletal patterning, limb development, and bone remodeling.
Dysregulation of BMP5 expression or signaling has been implicated in various skeletal disorders, including osteoporosis, osteoarthritis, and skeletal dysplasias. BMP5 mutations or alterations in its expression levels can disrupt normal bone development and contribute to skeletal abnormalities. Additionally, BMP5 has been found to play a role in cancer progression. Depending on the context, BMP5 can act as either a tumor suppressor or a promoter by influencing cell proliferation, apoptosis, angiogenesis, and metastasis.
Despite significant progress, several challenges remain in BMP5 research. Understanding the precise mechanisms underlying BMP5 signaling and its cross-talk with other signaling pathways is crucial. Additionally, investigating the context-dependent functions of BMP5 in different tissues and disease conditions is important for developing targeted therapies. Future research should focus on elucidating the regulatory networks controlling BMP5 expression and identifying novel therapeutic targets within the BMP5 signaling pathway. Moreover, studying the potential of BMP5 as a diagnostic or prognostic marker in various diseases would further expand its clinical relevance.
The expression of BMP5 is tightly regulated through a combination of transcriptional, post-transcriptional, and post-translational mechanisms. Transcription factors, such as Smads, homeobox proteins, and other tissue-specific regulators, bind to the promoter region of the BMP5 gene, modulating its expression. Additionally, microRNAs and RNA-binding proteins can influence the stability and translation of BMP5 mRNA. Furthermore, epigenetic modifications, including DNA methylation and histone acetylation, play a role in regulating BMP5 expression during development and in response to environmental cues.
Targeting BMP5 signaling holds therapeutic potential for various diseases. For example, in bone-related disorders, modulating BMP5 activity could be explored to promote bone regeneration or inhibit excessive bone loss. In cancer, therapeutic strategies targeting BMP5 signaling could be designed to regulate tumor growth, metastasis, or sensitize cancer cells to existing therapies. However, further research is needed to fully understand the complex role of BMP5 in disease pathogenesis and to develop targeted therapies that specifically modulate BMP5 activity.
Customer Reviews (3)
Write a reviewRevolutionizing the art of experimentation, this reagent blazes a trail of innovation.
By using this reagent, I am able to delve deeper into my research topics, deriving more persuasive conclusions.
The reagent showcases remarkable versatility and unwavering stability when it comes to the manipulation of intricate samples.
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