Recombinant Rat Cd40lg, Fc-tagged

• Cat.No.: Cd40lg-8725R
• Product Overview: A DNA sequence encoding the rat CD40LG (Gly115-Leu260) was expressed with Fc region of human IgG1 at the N-terminus.

Specification

• Description: CD40 ligand, also known as CD40-L, T-cell antigen Gp39, TNF-related activation protein, Tumor necrosis factor ligand superfamily member 5, CD154, CD40L and TNFSF5, is a single-pass type II membrane protein which belongs to the tumor necrosis factor family. CD40L ( CD154 / TNFSF5 ) is specifically expressed on activated CD4+ T-lymphocytes. The soluble form of CD40L ( CD154 / TNFSF5 ) is derived from the membrane form by proteolytic processing. N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40. Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. CD40L ( CD154 / TNFSF5 ) mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. It is involved in immunoglobulin class switching. Defects in CD40L are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1), also known as X-linked hyper IgM syndrome (XHIM). It is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes.
• Source: Human Cells
• Species: Rat
• Tag: Fc
• Predicted N Terminal: Glu
• Form: Lyophilized from sterile PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Molecular Mass: The recombinant rat CD40LG comprises 406 amino acids and predicts a molecular mass of 44.4 kDa. The apparent molecular mass of the recombinant protein is approximately 53 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: 1.0 eu/μg of the protein as determined by the lal
• Purity: >80 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: Cd40lg CD40 ligand [ Rattus norvegicus ]
• Official Symbol: Cd40lg
• Synonyms: CD40LG; CD40 ligand; CD40-L; CD40 ligand CD154; tumor necrosis factor ligand superfamily member 5; tumor necrosis factor (ligand) superfamily, member 5; Cd40l; Tnfsf5
• Gene ID: 84349
• mRNA Refseq: NM_053353
• Protein Refseq: NP_445805
• Pathway: Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem
• Function: CD40 receptor binding; CD40 receptor binding; CD40 receptor binding; cytokine activity; molecular_function; tumor necrosis factor receptor binding