Recombinant Human NCK2, His-tagged NCK2-15879H

Recombinant Human NCK2, His-tagged

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Recombinant Human NCK2, His-tagged

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Cat.No. : NCK2-15879H
Product Overview : Recombinant human NCK2 protein, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Description : NCK2 is a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptiona splice variants, encoding different isoforms, have been characterized.
Source : E. coli
Species : Human
Tag : His
Form : Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT
Molecular Mass : 45.3 kDa (403aa) confirmed by MALDI-TOF
Protein length : 1-380aa
AA Sequence : MGSSHHHHHH SSGLVPRGSH MGSMTEEVIV IAKWDYTAQQ DQELDIKKNE RLWLLDDSKT WWRVRNAANR TGYVPSNYVE RKNSLKKGSL VKNLKDTLGL GKTRRKTSAR DASPTPSTDA EYPANGSGAD RIYDLNIPAF VKFAYVAERE DELSLVKGSR VTVMEKCSDG WWRGSYNGQI GWFPSNYVLE EVDEAAAESP SFLSLRKGAS LSNGQGSRVL HVVQTLYPFS SVTEEELNFE KGETMEVIEK PENDPEWWKC KNARGQVGLV PKNYVVVLSD GPALHPAHAP QISYTGPSSS GRFAGREWYY GNVTRHQAEC ALNERGVEGD FLIRDSESSP SDFSVSLKAS GKNKHFKVQL VDNVYCIGQR RFHTMDELVE HYKKAPIFTS EHGEKLYLVR ALQ
Purity : >85% by SDS - PAGE
Applications : SDS-PAGE
Storage : Can be stored at 4°C short term. For long term storage, aliquot and store at at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration : 0.5 mg/ml (determined by Bradford assay)
Gene Name : NCK2 NCK adaptor protein 2 [ Homo sapiens ]
Official Symbol : NCK2
Synonyms : NCK2; NCK adaptor protein 2; cytoplasmic protein NCK2; NCKbeta; SH2/SH3 adaptor protein NCK-beta; growth factor receptor-bound protein 4; noncatalytic region of tyrosine kinase, beta; GRB4;
Gene ID : 8440
mRNA Refseq : NM_003581
Protein Refseq : NP_003572
MIM : 604930
UniProt ID : O43639
Chromosome Location : 2q12
Pathway : Activation of Rac, organism-specific biosystem; Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Downstream signal transduction, organism-specific biosystem;
Function : cytoskeletal adaptor activity; protein binding; receptor signaling complex scaffold activity;

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